| Dairy cows postpartum were in an oxidative stress state,oxidative stress will produce excessive free radicals and lipid peroxidation,excessive free radicals on cells and tissues can cause immune decline and inflammatoryresponse inside body,lipid peroxidation can lead to fat metabolism disorders,Free radical damage lead to the body of negative energy balance,accumulation of high level of Ketone body can cause the ketosis,Polyphenols are natural antioxidants that effectively scavenge oxygen free radicals andprevent lipid peroxidation,enhance the body’s antioxidant properties.The aim of this study was to investigate the effects of tea polyphenols on the antioxidant capacity,ketone level,lipid metabolism of plasma and whey and production performance in early lactation.In this study 25 cows of first parity in early lactation were selected,and randomly divided into five groups,labeled TPO,TP 10,TP20,TP30,TP40 group,TPO is the control group,TP 10 group’s additive amount is 10mg/(kg*BW),the additive amount of TP20,TP30,TP40 groups were 20mg/(kg*BW),30mg/(kg*BW),and 40mg/(kg*BW),respectively.Blood samples were collected before and after the test.Plasma oxidative/antioxidative parameters(TAC,GPX,SOD,MDA,H2O2,NO),blood biochemical parameters(AST,ALT,GGT,TBil,TG,CHOL,TP,GLOB,ALB,UA,CREA,BUN,GLU),ketone bodies(KET)and nonesterified fatty acid(NEFA)were measured;Milk samples were collected every three days from begin of test to 3days after the test,in total of 8 times,dynamic monitoring whey oxidative stress parameters(TAC,GPX,H2O2),whey ketone bodies,free fatty acid and the Somatic cell count(SCC);and dairy cattle production performance(milk yield,milk quality)were measured and the data statistics was analyzed.Result,1.The dynamics of whey was similar to that of ketone bodies,NEFA,TAC and GPX,dynamic analysis found that whey ketone bodies、NEFA and TAC was significantly than the control group(P<0.05),During the test the Whey H2O2 levels of all groups were decreased,those of TP20,TP30 and TP40 were significantly decreased(P<0.05).2.Plasma comparative analysis before and after test,The plasma ketone level in TP20 and TP30 were significantly decreased(P<0.05),The level of NEFA in TP30 were significantly decreased(P<0.05);The TAClevel of TP20 wassignificantly increased(P<0.05)and that ofTP30 was very significantly increased(P<0.01),The level of GPX in TP30 and TP40 wassignificantly increased(P<0.05),Biochemical indexes smaller fluctuations were within the normal range,AST,GGT,BUN and CREA activity decreased,no significant differences。3.Milk SCC dynamic analysis found that the number of SCC in TP 10,TP20,TP30 group were decreased,Milk production in the test before and after TP30,TP40 group were increased by 12.8%and 20.55%,The le’vel of the Milk protein in TP20,TP30 and TP40 group were higher than thatin control group.The level of Milk fat in TP30 and TP40 were lower than that in control group.4.Correlation analysis showed that between plasma and whey,the KET had Moderate correlation(r=0.573,P<0.01),NEFA had low correlation(r=0.358,P<0.05),BUN had high correlation(r = 0.854,P<0.01),CREA had low correlation(r=0.411,P<0.01),TAC had low correlation(r=0.309,P<0.05).In conclusion,Tea polyphenol feeding for postnatal cows can improve energy metabolism,antioxidant properties and production performance of dairy cows.Able to regulate a small range of cows liver function and kidney function,TP30 and TP40 group additive amount is more obvious.In the future,we need to In-depth study on the number of dairy cows,the milk production efficiency and the economic benefit. |
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