Proteomic Study On The Root Of Wheat Under Salt Stress By AMF

Research Background :The influence of human activities on nature is shown in the following aspects: Ozone layer destroy ozone,Greenhouse effect enhancement,climate anomaly,Alien invasion.These factors lead to soil salinization,soil biodiversity and function will inevitably change,and then affect the sustainable development of agriculture,forestry and animal husbandry production and ecosystem productivity.Wheat is one of the most important food crops in China,which has important scientific research value.Arbuscular mycorrhizal fungi(AMF)are a kind of common endophytic fungi in nature,which can form symbiosis with more than 80% terrestrial plants.AMF infection of host plants to form a symbiotic,the hyphae have symbiotic network huge,can expand the host plant roots and soil contact surface area,promote plant absorption of mineral nutrition and moisture.To enhance the resistance of host plants to promote the growth and development of plants under adverse conditions,Test purposes:in order to achieve the purpose of comprehensive utilization of saline alkali land.This experiment is to understand the mechanism of AMF on Wheat Seedling under salt stress,the adaptation mechanism of plant response to environmental stress to add new content and provide experimental basis.At the same time,it also provides new ideas for the improvement of plant salt tolerance,the improvement of growth conditions and the improvement of plant varieties.Experiment method :In this study,the main cultivar Linfen 5030(Triticum aestivum Linn)was used as the research material.The effect of AMF on the physiological and biochemical indexes of Wheat Roots under different salt concentrations was studied,and the best AMF strains(Gm and Gt)were screened out,And the optimal treatment of salt concentration under AMF was carried out.Select the following groups : Group NG(without inoculation),group S(0.4%NaCl treatment),group Gt(with soil ratio: 30g/300g)and GtS group(0.4%NaCl and Gt treatment)and related proteins of four treatment groups were analyzed.By using 2-DE technology,MALDI-TOF-MS technology and bioinformatics analysis,the expression of the differentially expressed proteins in wheat roots was compared to explore their biological functions.The results are as follows :Test results:(1)Effect of AMF on wheat growth phenotype treated with different salt concentrations: With the increase of salt concentration,the growth inhibition of wheat was more obvious.However,Linmai 5030 treated with AMF had a great advantage in phenotype,and the plant length and root surface area showed an increasing trend and the difference was significant.(2)The infestation degree of wheat root treated with AMF for different salt concentrations,and the infection rate of Gt was 36.63%.The infection rate of Gm was 29.90%,so Gt has an advantage over Gm.(3)Determination of physiological and biochemical indexes of wheat roots treated with AMF at different salt concentrations: With the increase of salt concentration,the activity of antioxidant enzymes decreased in enzymatic detoxification system and MDA content increased.However,the activity of antioxidant enzymes in AMF was higher than that of inoculated plants.(4)Analysis of the total protein of wheat roots treated with AMF at different salt concentrations: Four kinds of proteins were identified.Namely,class Ⅰ,energy metabolism related proteins;type Ⅱ,glucose metabolism related proteins;class Ⅲ,amino acid metabolism related proteins,class IV,course of defense-related proteins.