Behavioral Characteristics And Attractant Study Of Two Species Of Anoplophora

Anoplophora glabripennis and A.chinensis are considered to be two of the most serious wood-boring pests.They often coexist and endanger the common hosts and share the same aggregation pheromones.This paper was designed to provide a simple method for identifying of two species of Anoplophora larvae through the larvae of the typical characteristics of the larvae pronotum and combined with molecular methods.The indoor feeding preference of A.glabripennis and A.chinensis adults on twigs of three host species,Acer negundo,Salix babylonica and Melia azedarach,was analyzed by using feeding area method,and we also studied oviposition preferences of A.glabripennis adults on the trunks of three host plants.And the components of the twigs and trunks of host plant volatiles were analyzed by using dynamic headspace adsorption combined with gas chromatography-mass spectrometry(GC-MS).The trapping effects of aggregation pheromone and plant volatiles on these two species were investigated by field trapping tests inYuyao and Cixi,Zhejiang.The main results turn out:1、Identification of the pronotum Morphological characteristics of two Anoplophora larvaeThrough the typical characteristics of the pronotum of the A.glabripennis and A.chinensis larvae,it is easy to distinguish between the two species of the Anoplophora larvae and to further verify the method by means of molecular identification.2、Studying on feeding preference of two species of AnoplophoraThe results of indoor choice and no-choice feeding experiments showed that the most preferred host plants of A.glabripennis were A.negundo,followed by S.babylonica,and then M.azedarach,while the preference order of A.chinensis to host plants was just opposite;the most preferred host plant was M.azedarach,followed by S.babylonica,and then A.negundo.3、Studying on oviposition preference of two species of AnoplophoraThe results of indoor choice and no-choice oviposition behavior experiments showed that the mostpreferred host plants of A.glabripennis was S.ohsidare,followed by M.azedarach and then A.negundo.The success rate of oviposition on S.ohsidare respectively was 69.36% and 84.44%.4、Analysis of volatiles of host plant branches by GC-MSAnalysis of the volatiles of host plant branch showed that,12,9 and 11 compounds were tested in the volatile compounds of the three host species,A.negundo,S.babylonica and M.azedarach.In the host plant twigs volatiles,there were many terpenoids and aromatic compounds,and each host species had several unique volatile components and there were significant differences in the volatile components among different host species,and styrene,cis-3-hexenylacetate and acetophenone were only tested in A.negundo;nonanal was only tested in S.babylonica;Camphene,cis-3-Hexen-1-ol,Ocimene and β-Caryophyllene were only tested in M.azedarach;and the contents of 3-carene in S.ohsidare and M.azedarach was significantly higher than A.negundo.5、Analysis of volatiles of host plant trunks by GC-MSAnalysis of the volatiles of host plant trunks showed that,A.negundo and M.azedarach test were measured 16 kinds of compounds,S.babylonica tested 11 kinds of compounds.The main components of volatiles in the host plant trunks were olefins and alkanes,followed by aldehydes and so on.The volatiles in different host trunks were significantly different,and 3-carene and 2-ethyl-Hexanal were only tested in S.ohsidare,and the contents of nonanal,styrene and acetophenone in S.ohsidare were significantly higher than M.azedarach and A.negundo.6、The trapping two species of Anoplophora in the fieldThe results of the field trapping test showed that the mixed formula MK(4-(n-heptyloxy)butan-1-ol+4-(n-heptyloxy)butanal+camphene+cis-3-hexen-1-ol+ocimene+β-caryophyllene)of the aggregation pheromones and M.azedarach volatiles,aggregation pheromone formula M(4-(n-heptyloxy)butan-1-ol+4-(n-heptyloxy)butanal),M.azedarach formula K(camphene+cis-3-hexen-1-ol+ocimene+β-caryophyllene)and S.babylonica formula L(nonanal)all trapped A.glabripennis and A.chinensis adults at the same time.Among them,the mixed formula MK of the aggregation pheromones and M.azedarach volatiles showed the best trapping effect on A.glabripennis and A.chinensis adults,and trapped more adults of both species than aggregation pheromones or plant volatiles alone.The proportion of females trapped by aggregation pheromones was higher than the proportion of males,while the proportion of males trapped by plant volatiles were higher than the proportion of females.