Effects Of Gut Microbes And Different Nitrogen-phosphorus Artificial Diets On Growth And Development Of B. Cucuribitae

B. cucuribitae is an important agricultural pest,the previous studies focusing on morphological characteristics, external environment and different bait trapping effect of the macro aspects of it,in the aspect of gut microbes has not been reported.Ecological stoichiometry has been main carried out in aquatic ecosystems,such as zooplankton.In order to explore the green control technology of B. cucuribitae,this study intends to carry out research from these two aspects to provide a new method for pest control.(1)3 kinds of bacteria were isolated from the gut of male adults, and there were 6 kinds of bacteria isolated from the gut of female adult,and gram staining was performed, all of which were gram positive bacteria.It was also confirmed that 700?g/ml Amp and Gen could be used to remove the culturable bacteria in the gut of male adults without affecting the feeding. The combination of lmg/ml Amp and Gen could remove the culturable bacteria in the gut of female adults without affecting the feeding.(2) The effects of Gen,Str and Amp on the removal of gut microbes from the larvae of B. cucuribitae were studied under the concentration of lmg/ml,among them Amp is best, Str is better, Gen is the weakest.And compare the three kinds of antibiotics after treatment, changes in weight, pupal weight and the contents of P in the larval.The effect on body weight and the content of P in the larvae have reached a significant, to the pupal weight, the effect of Str has no significant.(3) The experimental model of the three year old larvae of B. cucuribitae which had no culturable gut bacteria was obtained by the addition of antibiotics in the artificial diet. The composition of ampicillin, rifampicin, streptomycin and gentamicin mixture were used, a final concentration of 300 ug/ml. And compared to the weight,pupal weight and average emergence rate of the normal and antibiotic treatments of B.cucuribitae,the results showed that after antibiotic treatments, compared to the weight of three instar larvae of normal control, there was significant difference; while the pupal weight and average emergence rate had no significant difference. The contents of total N and total P in the three instar larvae treated with antibiotics had a great change, which was significant, but there was no significant difference between N:P.(4) The artificial feed with different nitrogen and phosphorus were designed,which were the conventional feed one and the conventional feed two. The changes of body weight, pupal weight and N, P in the three instar of B. cucuribitae larvae;developmental duration, P and RNA content in the B. cucuribitae larvae were compared after feeding different nitrogen and phosphorus diets.There was no significant difference in the weight and pupal weight between the three instar larvae feeding the conventional one and the conventional two. At the age of one, the development time of the two groups was the same, the content of P was the same too,but at the age of two and three, the development time of the conventional feed two was significantly shorter than that of the conventional feed one,the content of P was higher than that of the conventional feed one. The content of RNA feeding on conventional two was higher than that of the conventional one.