Genetic Mapping Of The Tiller Angle Gene In Barley

Barley?Hordeim vulgare L.?is the fourth largest cereals in the world,it's also the main crops in our country.The global demand of food will be double to 2050,in order to face this condition,the food production needs to increase by an average of 2.4% per year.Tiller number and tiller angle are two important agronomic traits for gramineous crops.Prostrate growth is the tiller angle increased largely in spreading type and the negative gravitropism disappeared or decreased.Crops prostrate growth will reduce the leaves cover each other,increase the cross-sectional area of light,then increase the energy use;Also it can cover the land surface,keep soil,water and nutrients,reduce the growth of weeds.Prostrate growth have influence on the crop grain yield.So it is important to mapping the prostrate growth gene in barley.The barley line V-V-HD,which shows prostrate tillers at vegetative growth stage and erect tillers after booting stage,is considered to be the ideotype to repress the growth of weeds at the early stage.In this study,a double haploid?DH?population from the cross of V-V-HD and Buloke was used to map the prostrate growth gene.Through the DH population trait data,we find the prostrate growth was controlled by a single gene.Through genetic analysis,the target gene is mapped on chromosome 3HL near the SNP marker 2₀343.So we find more SSR markers and design InDel primers near the marker 2₀343,finally the target gene was mapped to a 3.53 Mb region between the markers InDelz3028?631.84Mb?and InDelz3032?635.37Mb?.There are 52 genes in this region and divided into nine parts.This will help us better understanding the prostrate trait.The markers developed in the present study could be used for marker-assisted selection?MAS?in breeding projects.