Research Of Pseudolarix Amabilis Rehd Seed Germination And Rapid Breeding

The optimum conditions for germination of Pseudolarix amabilis Rehd was establishedby using the method of single factor test and orthogonal test,andthe fast breeding system of Pseudolarix amabilis Rehd was been established;The suspension culture and the rapid regeneration culture system of Pseudolarix amabilis Rehd cells was been established,too;The bestculture conditions of inducing polyploid seed successfully by Pseudolarix amabilis Rehd was been esplored;The endophytic bacteria was isolated from the fresh branches of Pseudolarix plants,and then,the antibacterial experiments were carried out,The results are as follows:(1)The effects of 1% carbendazim solution on the germination rate and the bacteria uptake rate were studied by single factor test.The results showed that-Along with the prolonging of the treatment time of the 1% carbendazim solution,-the seed germination rate and the infection rate declined.The most conducive to improving the seed germination rate of Pseudolarix and reduce the contamination rate was been treated for 1% carbendazim solution seed 3 hours,andthe seed germination rate was is(82 + 0.32)%,and the contamination rate was only(3 + 0.49)%.In addition,we also studied the effects of the different GA concentration,different temperature and different soaking time on the seed germination of Pseudolarix amabilis Rehd,the results showed that under the condition of 60 ?,6 ‰ of Gibberellin Treatedfor 2 hours,and then placed in a Petri dish for 30 days,-the highest germination rate of seeds of Pseudolarix amabilis Rehd was observed,andit was 97.33%.(2)The best medium for Pseudolarix amabilis Rehd hypocotyl explants was MS + 2,4-D1.0mg/L + 6-BA 0.5mg/L + KT 0.1mg/L;Except for hormones,the callus produced explant was related to the inoculation method and the explant cutting mode;there were six forms of callus in the process of the formation of culture,and the highest cell viability were the green,light green and light yellow callus.(3)The best medium MS 6-BA + 1.0mg/L + 2,4-D + 0.3 mg/L + 1.2 g/40 ml + sucrose40g/L inoculation p H6.0.In the process of suspension culture of Pseudolarix amabilis Rehd cell growth,the specific performance of the delay period was 0 ~ 3d,the index for a period was 3 ~ 7d,the decline period was 7 ~ 20d;In addition,it was observed that the growth rate of green callus was fast,the growth rate of yellow green and yellow calli was slow,and the brown change rate was fast in the experiment.(4)In the rapid regeneration of plant Pseudolarix,Pseudolarix leaves were selected as the explants for callus induction,the optimal medium for callus induction was GD + NAA 1mg/L + GA3 1 mg/L,the induction rate was 65.4% after 30 days of statistical callus culture,the best adventitious bud induction medium was MS + TDZ 0.2 mg/L Ad + 20 mg/L 35 d culture induction rate was 23.1%;the best medium for adventitious root induction was 1/3MS+ IAA 1 mg/L + activated carbon 30mg/L,cultured 35 d induction rate was 54%.After three days,the aseptic seedlings were planted in thegreenhouse,and the survival rate was about93%.(5)In the process of plant polyploid induction of Larch,the optimum induction culture conditions were under 30?,0.2% colchicine for 36 h,the induction rate was 78.63%;the minimum conditions of the low induction rate is 20?,0.1% colchicine for 12 h,the induction rate was 49.94%;When colchicine the concentration of 0.2%,time of colchicine action increased from 12 h to 36 h,its polyploid induction rate gradually increased,when the reaction time increased from 36 h to 48 h,the induction rate decreased.(6)Seven endophytic bacteria were obtained-from fresh Pseudolarix branch in this study,such as JJ18,JJ243,JJ314,JJ318,JJ416,JJ27,JJ46;Staphylococcus aureuswas used as the indicator bacteria,Candida albicans,Trichophyton rubrum,Saccharomyces cerevisiae and Aspergillus niger and the MIC(minimal inhibitory concentration)and the MBC(minimum bactericidal concentration)of various bacteria were tested.