| In this experiment,we chose three hundred and sixty Hy-line Brown later-laying hens whose laying egg rate and the physical conditions is similar.The age of the hens is 383.We divided the Hy-line Brown hens into two groups,and we set three repeat groups in each experiment group.We fed the hens of blank group with normal diets,we added the antibacterial peptide which concentration is 0.01L/kg into the diet of experiment group.The experiment cost forty-two days,we spent seven days in pre-feeding experiment and we spent thirty-five days in breeding experiment.We determined the product ability,the biochemical level in the serum,the nutrition of egg,discuss the antibacterial peptide how to influence the product ability of Hy-line Brown hens and the cholesterol in the egg.We analysis the data and then we research the influence of antibacterial peptide in genes level.We determine the expression level of HMGCR m RNA and Apo-Ⅱ m RNA with the technology of RT-PCR.We do this experiment aim to make the product ability higher and improve the benefit of layer industry,and make the lowing-cholesterol egg production possible.The following is the data of the experiment.1.The product ability: the laying rate of experiment group is 7.07% significantly higher than the blank group(P<0.05).The broken egg rate,egg weight and the feed-egg ratio is 12.19%,0.96%,0.84 lower than blank group,but there isn’t significant difference(P>0.05).2.The biochemical level in the serum: the total protein of experiment group is significant 7.2% lower than blank group(P<0.05),and the LDL of experiment group is significant 12.6% lower than blank group(P<0.05).The ALB and GLB of experiment is 0.82% and 9.48% lower than blank group,the TC and TG of experiment group is 4.39% and 20.16% lower than blank group,the blood glucose decrease 4.09%,but there isn’t significant difference(P>0.05).3.The nutrition of egg: the cholesterol in the egg of experiment group is significant 18.61% lower than blank group(P<0.05).All of the amino acid in the egg of experiment group is higher than the other group,the total amino acid increase 6.79%,but the difference was not significant(P>0.05).the cholesterol in the egg was significantly decreased by.18.61%.The quantity of P,Mg,Zn,Se in the egg increase and the quantity of Ca decrease.4.Expression level of HMGCE and Apo-Ⅱ: compared with the control group,the expression level of HMGCR in livers was significantly decreased(P<0.05)and the expression of Apo-Ⅱ was decreased,but the difference was not significant(P>0.05).Conclusion:1.Antimicrobial peptides significantly increased laying rate 7.07%,improve the product ability performance of Hy-Line Brown hens.2.Antibacterial peptide significantly decrease the serum total protein and LDL-C.3.Antimicrobial peptides increased the amino acid in the egg,and significantly decrease the quantity of cholesterol in the egg 18.61,optimize the nutrition of the egg.4.Antibacterial peptide could significantly improve the expression level of HMGCR m RNA in livers of Hy-Line Brown hens... |
