| Citrus is the most important fruit tree in the world,due to a longer childhood,citrus breeding has been seriously hampered.In addition,citrus is a typical multi-embryo plant,there is the phenomenon of nucellular embryo,so sexual hybrid breeding has a serious impact on the consistency of seedlings.In the actual production of grafting technology can effectively shorten the childhood to avoid the reproductive propagation of the late flowering,the beginning of the fruit is late,rich period of delay and so on.The harvest fruit of the grafted seedlings can be shortened from 6-8 years to 3 to 5 years,and the graft propagation can choose different rootstocks as needed to improve the quality of the fruit and increase the fruit yield,so that the same batch of seedlings Physiological growth habits are highly consistent.In this study,climantine orange was grafted onto the clones of clenbuterol and the ungrafted clones.They were compared by transcriptome sequencing after bioinformatics analysis.The mRNA expression of the scion and rootstocks was observed by using the specific SNPs between the scion and the stock as markers.The main results are as follows:1、The transcriptome sequencing analysis shows that genes are differentially expressed in the presence of citrus grafted rootstock and scion.And the effect of gene expression on rootstocks was more pronounced than scion.Compared with the control group,the expression of 598 genes was significantly up-regulated and the expression of 993 genes was significantly down-regulated.Compared with the control group,only 53 genes were up-regulated and 69 genes were significantly Down-regulated.This shows that the effect of grafting on the expression of gene of scion was lower than that of rootstock;2、Comparative analysis showed that there were 563 stock specific SNP in the scion,and 1268 scion’s SNP were found in stock;3 stock’s InDel were detected in scions,and 33 scion’s InDel were found in stock..The results showed that there were SNP and InDel changes between the stock and the scion on the grafted plants.In the grafted plants,either SNP or InDel,the change is always rootstock better than the scion,the number of moving always downward better than upward.3、Using SNP as a label,the movement of mRNA in grafted plants was determined by the change of SNP before and after grafting of mRNA in the rootstock and scion tissue.It is found that there are 452 mRNA in the upward moving SNP,960 mRNA in the downward movement of SNP,only 16 mRNA whith the upper and lower SNP.The results showed that there were mRNA mobile exchanges between graft and stock after grafting.Moreover,the upward movement of mRNA is less than the downward movement of mRNA,at the same time,a small number of mRNA can move up and down between the stock scions without direction;4、After verification,there can be mobile mRNA gene in Citrus Clementina roots,stems,leaves and flowers for gene expression test.It is found that the movement of mRNA in genes is not related to the specific expression of genes in different tissues.Some genes have the highest expression in flowers or roots.Since gene expression may be related to the function involved in it,it is inferred that some genes may be synthesized at the leaves or roots but are involved in the process of life in flowers,so this phenomenon occurs. |
