| Dehydroepiandrosterone(DHEA)is synthesized by the zona reticularis of adrenal cortex and to a lesser degree with the gonads(testicle and ovary).The conjugate sulfate(DHEAS)is the main form in the circulating blood.DHEA is a precursor of sex steroid hormone biosynthesis.DHEA can transfer to estrogen,androgens and corticosterone with steroidogenic enzymes in peripheral tissues.DHEA has numerous biological and biochemical effects,especially on the lipid metabolism,but the lipid mechanism of SD rats has not been clarified,so this test was focus on the lipid metabolism rat with exogenous DHEA.The purpose is to provide theoretical and experimental evidence for regulating the lipid metabolism of rat.1.Long-term administration of DHEA on lipid metabolism in rats feed with normal dietThis study was aim to investigate the effect of DHEA on Conventional biochemical indicators and lipid metabolism related gene expression.60 male SD rats were randomized into 4 groups:control group(o mg/kg·BW DHEA),low-dose group(25 mg/kg·BW DHEA),middle-dose group(50 mg/kg-BW DHEA)and high-dose group(100 mg/kg·BW DHEA).The result showed that DHEA significantly decrease body weight in middle-dose group when compared with control group(P<0.05).Serum triacylglycerol and glucose levels were high significantly decreased in middle-dose group.High-density lipoprotein was significantly increased in high-dose group.Fat synthesis related gene determination results showed that FAS mRNA expression was significantly decreased in middle-dose group when compared with control group.SREBP-1 and HNF-4 mRNA expression were significantly decreased in every DHEA group.However,ACC mRNA expression showed no significant change.Adipolysis related gene determination results showed that ACO mRNA expression was significant decreased in every DHEA group.LCPT-1 and PPARa mRNA expression were significant decreased in low-dose and high-dose group.ATGL mRNA expression was significantly decreased in middle-dose and high-dose group.These findings indicated that DHEA affects the key factors of lipid metabolism result in decreasing the fat deposition on male rats.2.Long-term administration of DHEA prevented the fat deposition in rats with fed high-fat dietThe aim of present study was to identify the effects of dehydroepiandrosterone(DHEA)on lipid metabolism parameters and lipogenic gene mRNA expression in rats with fed high-fat diet.A total of 75 rats were randomized into normal diet group(NCG),and high-fat diet with 0(HCG),25(HLG),50(HMG),100(HHG)mg/kg·body weight DHEA group.All rats were treated with DHEA or vehicle via gavage one time per day for 8 weeks.Gavage DHEA could inhibit body weight in rats with high-fat diet,and it was significant decreased in HMG group when compared to HCG group.No statically difference was observed,while the relative weight of epididymal fat showed a decreased tendency in DHEA-treated group than that HCG group.Hepatic triglyceride and total cholesterol contents were significant decrease in HMG group and HHG group,while serum high-density lipoprotein content in HMG group and in HHG group was significant increase when compared to HCG group.Hepatic lipase activity in the HMG group was significant higher than that of HCG group.FAS mRNA level was markedly decreased in the HLG and HHG group,and SREBP-1 mRNA level was significant decrease in the HMG and HHG group when compared to HCG group.ACO and LCPT-1 mRNA abundance were significant decreased in HLG and HHG group when compared to HCG group.ATGL mRNA abundance was unaffected in the DHEA-treated group that HCG group,whereas HSL mRNA level was significant increase in the HMG group than that of HCG group.These findings demonstrated that long-term administration of DHEA could reduce the synthesis of endogenous triglycerides by inhibiting SREBP-1 and FAS mRNA levels,and it also could accelerate the lipolysis of exogenous triglycerides through enhancing expression of ATGL and HSL mRNA levels,which eventually reduce fat deposition in rats with fed high-fat diet. |
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