Study On The Efficient Rapid Propagation Technology Of Dendrobium Officinale

Dendrobium offcinale Kimura et Migo is a perennial herbaceous plants,also known as huangcao,also called heijiecao,and it is our country unique rare Chinese herbal medicine.D.officinale contains polysaccharide,amino acid,alkaloids,trace elements and other nutrients,has the very high application value in medicine.But the D.officinale often pick on the cliffs,and in exalbuminous seeds,it is hard to germination under natural conditions,so wild D.officinale resources are drastically reduced.In order to protect this rare traditional Chinese medicine,using modern science and technology has been very important.This article mainly use D.officinale seeds and stem as explants,protocorm induction,proliferation,differentiation and root seedling tissue culture conditions were discussed,in order to establish a complete set of D.officinale rapid propagation method.The main results were as follows:1.With D.officinale seeds as explant,by adding different additives induce protocorm directly.It was concluded that the best medium for D.officinale seed germination was:1/2 MS + 1.0 mg/L NAA + peptone 2.0 g/L + sucrose 25 g/L + agar 7.0 g/L + 1.0 g/L active carbon.On this medium,after 45 days of induction,D.officinale seeds grew up to be the protocorm with dark green color.Adding different concentrations of plant growth regulator,and through the combination of different concentrations of plant growth regulator to make D.officinale protocorm proliferation.Through experiment,it was concluded that the optimal culture medium for proliferation of protocorm:1/2 MS +1.0 mg/L 6-BA + 0.5 mg/L NAA +sucrose 25 g/L + agar 7.0 g/L+ 1.0g/L active carbon.The highest multiplication factor is 23.After proliferation,the protocorm was carry on differentiation cultivation.After adding different concentrations of plant growth regulator combination,found the best differentiation medium for D.officinale protocorm was:1/2 MS + 2.0 mg/L 6-BA+ 0.2 mg/L NAA + sucrose 25 g/L + agar 7.0 g/L + 1.0 g/L active carbon The average differentiation rate was high up to 95%.Rooting induction of the regenerated shoots was carry on after differentiation cultivation.Through experiment,it was concluded that the D.officinale best rooting medium for cluster bud was:1/2 MS + 0.3 mg/L NAA + 100 g/L mashed potatoes+ sugar 25 g/L + agar 7.0 g/L+ 1.0 g/L active carbon.Rooting rate was 100%.2.It is a method of rapid propagation with annual D.officinale stem with bud induction axillary bud experiment,adding different concentrations of 6-BA and NAA,TDZ,such plant growth regulator.It was concluded that the best medium for D.officinale stem with bud induction axillary bud was:1/2 MS + 6-BA 2.0 mg/L + 0.2 mg/L NAA + sugar 20 g/L+ 1 g/L active carbon+agar 7.0 g/L.Induce axillary bud taking root,adding different plant growth regulator,and promote the gr-owth of D.officinale seedlings Through experiment,it was concluded that the D.officinale best rooting medium for axillary bud was:1/2 MS + 0.3 mg/L NAA+ 10+ 100g/L mashed potatoes+ sugar 25 g/L + agar 7.0 g/L +1.0 g/L active carbon.Rooting rate was 100%.