Research About Interaction Mechanism Between Chinese Cabbage And Turnip Mosaic Virus

TuMV,a main pathologeny of plants,is one primary pathogen of Cruciferae in China.It can lead substantial losses in the yield and quality of Chinese Cabbage and non-heading Chinese cabbage.IChinese Cabbage and non-heading Chinese cabbage are native to China.With its unique flavor,the nutrient-rich vegetable is not only one of most important vegetable in Asia,it has gradually been loved all around the world.In recent years,researchers speculated that the 6K2 vesicles is the place for viral replication,and the vesicle formation and movement are directly related to the secretory pathway of the host cell and the SNARE proteins.In this study,Chinese Cabbage was used as materials to analyzes the role of membrane system played in host-virus interactions and the response of SNARE family under virus infection.Our research has some practical meaning to molecular breeding for disease resistance and academic significance to the interaction between TuMV and Chinese cabbage.At the same time,non-heading Chinese cabbage was used as materials to analysis of the protein components of TuMV 6K2 vesicles,which would provide a theoretical foundation for understanding of the mechanism of viral replication and the interaction mechanism between non-heading Chinese cabbage and TuMV.1.We performed a global analysis of gene expression in Brassica rapa and identified a a total of 3,470 differentially expressed genes in response to TuMV infection,including 2,013 upregulated genes and 1,457 downregulated genes.Among the complex array of changes affecting pathogen response,thirty-eight secretory pathway genes were identified.The quantitative real-time polymerase chain reaction results were consistent with microarray analysis.Combined with previous studies,we speculated that when RNA viruses interact with the host,viruses,or their components,use the host secretory pathway to accomplish viral replication and movement.We speculated the location of the secretory pathway genes.Exploring the precise function of the genes identified in this analysis will offer new insights into viral defense in B.Rapa and plant-virus interaction.2.Through bioinformatics and homologous analysis,proteins of SNARE family were identified from the database of the Chinese cabbage genome.Based on protein structure,the SNARE family proteins in Chinese cabbage was classified into two subfamilies,including R-SNAREs and Q-SNAREs.The identification,classification,phylogenetic tree,conserved motif,distribution on chromosomes were predicted and analyzed.We also analyzed the expression patterns in six tissues and the expression level under TuMV-infection.It was necessary and useful for us to comprehensive analysis of physiological functions and biological roles of SNARE family genes in Chinese cabbage,which will provide us rich resources and opportunities for the research of the interaction mechanism between virus and hosts,and improving the genetic characteristics of Chinese cabbage.3.In this study,TuMV resistance survey of 102 species non-heading Chinese cabbage was conducted.Selecting the susceptible varieties "Xiangqingcai" for the material,after TuMV infection,co-immunoprecipitation,protein electrophoresis and mass spectrometry analysis were used to separate 6K2 vesicles and systematic analysis of its protein components.Finally 15 viral replication associated proteins were isolated,which contains the heat shock protein 70,ATP enzyme,luminal binding protein,enolase,EF-Tu,and an unknown protein.This research provided the basis for understanding the mechanism of viral replication,interaction mechanisms between virus and hosts,and exploring new resistance genes.