| MEF2 (Myocyte Enhancer Factor 2) is a member of MADS-Box transcriptional factors. In vertebrates, MEF2 genes are multi-gene family, including MEF2A, MEF2B, MEF2C and MEF2D. MEF2 genes are expressed in smooth, skeletal, and cardiac muscle cells, which play essential roles in muscle differentiation and activate the genes expression involved in muscle differentiation, regenerate of muscle.In this study, The full length cDNA of MEF2 genes in Monoperus atbus were successfully cloned by common PCR, homologous cloning and we quantified MEF2 genes expressions in different tissues and different developm stages of skeletal muscle by real-time PCR, meanwhile we focused on frozen section and SDH enzyme histochemistry analysis of skeletal muscle regeneration, quantified MEF2 genes expressions in skeletal muscle regeneration. Results are as followed:The full length cDNA of MEF2 in Monoperus atbus were cloned, and sequences were deposited in the GenBank with accession numbers of KM103279, KM103280, KM103281 and KM103282. The length of MEF2 family were 1593bp,1419bp,1864bp and 2412bp, coding 482,472,477 and 535 amino acids. All of MEF2 genes contained the MADS-Box and MEF2 domains.The expression of Monoperus atbus MEF2 was reveal that these genes were expressed in different tissues and different developm stages of skeletal muscle at different levels.The expression levels of MEF2 in different development stages of skeletal muscle showed up-regulated with the developmental stage, especially MEF2C. The MEF2 genes expressed in skeletal muscle were significantly higher than in other tissues (p<0.05).The MEF2A and MEF2D were expressed highest in skeletal muscle followed by heart, lowest in other tissues.In this study, we established muscular injury model. The skeletal muscle’s regeneration after injured were obvious observed by frozen section and SDH enzyme histochemistry. The expression of MEF2 genes were appeared up-down than return to the normal level before injured. |
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