| Erysipelothrix rhusiopathiae bacteria are zoonotic pathogens, broad host range. Most harmful to pigs. At present, for its pathogenicity, bacterial surface protein struc-ture, characteristics and mechanism of action poorly understood.The experiment based Erysipelothrix rhusiopa thiae bacteria FUJISAWA gene sequence by bioinformatics Screening surface protective antigen protein 7, including sialidase, hyaluronidase. ABC transporter protein, ABC transporter binding protein, pectin lyase, choline binding protein,β-galactosidase.Using molecular cloning and prokaryotic expression cloning experiment express these seven proteins, So long as some fragments of protein expression segment is divided into 16 recombinant proteins. Binding by ELISA and immune mice immunized experiment alevaluation of the effect, selected recombinant protein S2 sialidase protein, recombinant β-galactosidase protein B2, B3 certain immune protection. Found β-galactosidase recombinant protein B2, B3 protection rate of 17%, ABC transporters and ABC-ATP binding transporter protein has the effect of a delay time of death, in mice after infection. |
