Genetic Analysis And Rough Mapping Of Recessive Genic Male Sterility14 (ms14) Gene In Maize

Maize genic male sterility mutant lines are one kind of elite germplasm, which is helpful to genetics and breeding research and hybrid production in maize.In the study, a maize genic male sterility mutant, male sterilityl4 (msl4) was preliminary studied.Firstly, phenotypical analysis and I2-KI dyeing of ms14 tassels and anthers shows that ms14 mutant is completely pollen sterile and exerts no anthers.Secondly, genetic analysis using two F2 segregation populations of msl4(msl4× zheng58 and ms14×chang7-2), the ratio of fertile to sterile plants is 3 to 1, which shows that the ms14 mutant is controlled by a recessive genic male sterility gene.Thirdly, through the linkage analysis between ms14 locus and molecular markers in the F2 segregation populations of ms14 (ms14×chang7-2), the ms14 gene is roughly mapped to an interval on Chromosome 1 between SSR marker umc2025 and umc1676, with the genetic distance of 2.2 cM and 0.3 cM, respectively.Finally, the potential application value of the ms14 gene in maize hybrid breeding and production is discussed.