The Analysis Of Soil Microbial Diversity Of Several Familiar Shrub In Shaanxi Province

To study the relationship between microbial diversity and the kinds of the shrub, the present research study 11 common shrub soil for soil microbial diversity. Shrubs mainly contain Grewia biloba, jujube, mayberry and beautiful shrub lespedeza,which located in Chang'an District of Xi'an Qinling.SopHora davidii, Ostryopsis, Vitex shrub, which located in Tongchuan City, Myrsine, forsythia shrubs, which located in Shaanxi Province Shirakawa iron County, and Salix shrub, artemisia ordosica, which located in Jingbian County, Shaanxi province, this study with the 11 kinds of common shrub soil research. Respectively, this research studied the physical and chemical properties,enzymatic activity, the number of microorganisms which can be cultured, biomass carbon and nitrogen, microbial diversity and the relationship between them,for providing basis theory of brush protecting and restoration.The results showed that:1) different shrub soil pH, soil moisture, soil enzyme exists a significant difference (P<0.05), there exists significant correlation between soil and shrub, with soil depth overall showing a downward trend; 2) different kinds of Shaanxi shrub soil microbial population are quite different. But each shrub with the largest number of bacteria, and the least fungi number, deep soil microbial quantity as a decreasing trend, between microbes and shrub species exists significant correlation; 3) biomass carbon and nitrogen of different shrub soil exist significant differences, different layers of the same shrub also exist significant differences, with depth biomass carbon and nitrogen showed a decreasing trend overall. Biomass carbon and nitrogen and shrub species were significantly correlated (P<0.01); 4) by PCR-DGGE analysis showed that there were significant differences in microbial diversity between different shrubs, but the effect of shrub species to soil microbial diversity is no significant.