Development Of The Wheat Lines Harboring The Alien HMW-GSs From Aegilops Sharonensis

In our previous work, we identified and cloned a novel novel high-molecular-weight glutenin subunits (HMW-GSs) from Aegilops sharonensis, a diploid relative of wheat, and the results indicated that HMW-GS encoded by Glu-ISsh has huge molecular weight of 2.93kb for x-type gene. Hybrids betweent tetraploid or hexaploid wheats and Ae. sharonensis have been successfully produced by wild crosses. And some of hybrid seeds were backcrossed with common wheat. In this study, we conducted backcrosses sequentially for 2 years (2013 and 2014). investigated of all derived lines. For all derived lines from backcross to wheat and selfcross, HMW-GS composition were checked by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and the agronomic traits were also investigated. Chromosome observation was conducted for lines with Aegilops HMW-GSs.The agronomic traits and HMW-GS component of backcross-derived lines vary continually in two years. The results of cytogenetics indicated that lines carrying Aegilops HMW-GSs had varied chromosome numbers ranging from 35 to 50. Of these lines, three ones with 42 chromosomes (25-8,33-1, and 42-2) were identified by cytogenetic observation.. The results of agricultural traits investigation showed three lines were highly similar to common wheat in morphology, together with possessing alien HMW-GSs of Ae. sharonensis and stable chromosome number, these three lines have potential value in wheat breeding. Additionally, we developed two molecular markers specific to the x-and y-type HMW-GSs genes of Ae. sharonensis by sequence comparison. These two HMW-GS gene specific markers were validated in accurately tracing and distinguishing Ae. sharonensis Glu-1Ssh of backcross progenies from Glu-IA, Glu-IB, and Glu-ID of wheat Therefore, the Glu-1Ssh HMW-GSs lines with 42 chromosomes, will be efficiently applied in wheat breeding by molecular assisted selection with gene-specific markers.