The Primary Study Of The Effect Of MADS-box Transcription Factors On Strawberrry Fruit Ripening By ABA

Fruit ripening and quality forming is complex, due to a variety of factors. Hormone and transcription factors are two key factors. Hormones and transcription factors are not independent in the regulation of fruit ripening, however, related report is limited. It has learned that ethylene is one of the important hormones regulating fruit mature, but its role is more positive in the non-climacteric fruit. and ABA plays an important role in climacteric fruit. At the same time, it has been confirmed that MADS can regulate buth non-climacteric fruit and climacteric fruit ripeing. It can regulate climacteric fruit ripeing by production and releasing of ethylene. Strawberry, as a typical climacteric fruit, is regulated probably by abscisic acid. So what is the relationship of MADS and ABA would be further studied.In this experiment, we use different concentrations of exogenous abscisic acid sparying different development (green, white, red) of strawberry fruit, and study the effect of ABA on strawberry ripening and quality in order to discuss whether ABA can promote strawberry maturing by promogting its quality. At the same time, we utilize different concentrations of abscisic acid sparying green phase of strawberry fruit, and pick the fruit every three days to study the development of endogenous abscisic acid content and quantity of FaMADSl, FaMADS2 gene, in order to discuss the relationship of MADS and abscisic acid content.Firstly,the syudy fouind that abscisic acid can promote strawberry fruit ripening and 30 mg/L abscisic acid spraying the green phase of strawberry fruit,35 mg/L abscisic acid spraying white fruit ripeness, and 40 mg/L abscisic acid spraying red strawberry fruit have the best effects, promoting ripening about 1 week ahead. Secondly, in terms of fruit quality, abscisic acid has no obvious influence on fruit weight and soluble solid. But 25mg/L-35mg/L ABA can promote the content of ascorbic acid,25mg/L-40mg/L ABA can promote the content of titratable acid. 25mg/L--30mg/L ABA can promote the fruit oxidation resistance, and 20mg/L-40mg/L ABA can promote anthocyanin accumulation. Therefore, abscisic acid can improve the quality of strawberry fruit, at the same time, can promote fruit ripenging. Compared with each treaments. it is found that 30 mg/L abscisic acid spraying green strawberry has the best effect.Abscisic acid can improve the content of endogenous abscisic acid in strawberry and NDGA treatment can reduce the endogenous abscisic acid content. Along the development of strawberry fruit. FaMADS1 gene expression quantity has the same trend as the trend of abscisic acid, and ABA can increase FaMADS1 gene expression, while NDGA inhibites FaMADSl gene expression. At the same time, in the strawberry fruit, endogenous abscisic acid content and quantity of FaMADS1 gene expression are extremely significant correlation. Therefore, it is inferred that ABA can regulate strawberry fruit ripening in the upriver of FaMADSl. On the other hand, FaMADS2 gene expression quantity is defferent from the trend of endogenous abscisic acid content, and low concentration of exogenous abscisic acid, NDGA can not promote FaMADS2 gene expression compared with control, but high concentration of ABA can promote FaMADS2 gene expression significantly. At the same time, the strawberry fruit endogenous abscisic acid content and FaMADS2 gene expression are significant correlation. Therefore, in the regulation of strawberry mature pathways, ABA and FaMADS2 may have some unkown relationgship.