| In this research, copper poisoning model were established to further discussingdifferent level of copper on rat hepatocyte proliferation activity, free radical metabolism and the membrane potential. The copper sulfate(Cu SO4)was used as the source of copper, there were six levels of different copper(Cu2+): 800 μg/m L, 400 μg/m L, 200 μg/m L, 100 μg/m L, 50 μg/m L, 0 μg/m L. In all of those groups, rat hepatocytes were cultured for 24 hours and 48 hours respectively, and the free radical produce, antioxidant enzyme activity and cell membrane potential were detected. The results showed that the concentration of copper was 400μg/m L and 800μg/m L, the cell proliferation activity decreased obviously,and the difference was significant(P<0.05). When concentration of copper below 400 μg/m L, the copper concentration and T-SOD activity were positively correlated, the same to GSH-Px, CP, CAT activity, and that was negatively correlated to the content of MDA and NO. The results were reverse when the concentration of copper were 400 μg/m L and 800 μg/m L, With the increase of copper concentration, the membrane potential decreased(P<0.05). The above results showed that the proper concentration copper could promote the antioxidant enzyme activity of cells, but high copper might result in a decreasing of enzyme activity, more free radical production, and the decreasing of the hepatocyte membrane potential. |
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