Preclinical Study On Huangqi Jingye San

Objective To develop the new medicinal part of Radix Astragali, to use the natural resources of the Stem Leaf of Radix Astragali reasonably, to lay the theoretical foundation in veterinary medicine field and to provide a scientific basis for its medicinal value, the Preclinical study on Huangqi Jingye San has been made.Methods(1) Study on quality standard of the Stem Leaf of Radix Astragali raw material medicine: the identification, paraffin section method and the microscopic identification method was used to study its character and organizational characteristics. A method of TLC identification was used for qualitative discrimination. The moisture, total ash and extracts contents of the Stem Leaf of Radix Astragali were determined by pharmacopoeia method. The astragaloside IV and calycosin-7-O-?-D-glucoside contents were determined by HPLC. Total contents of astragaloside and astragalus polysaccharide were measured by Colorimetry.(2)Study on the pharmaceutical research of Huangqi Jingye San: Prescription and preparation technology was determined by pharmacopoeia method. The quality standard was resolved by the single screening method, drying method and the load difference check method. According to the veterinary drug stability test specification regulation for the preliminary stability were studied.(3) Study on the pharmacodynamics research of Huangqi Jingye San: The times than the dilution and the drug sensitive slips method is adopted for the aqueous extracts and alcohol extracts of Huangqi Jingye San in vitro bacteriostasis experiment research. Through ConA and LPS induced multiplication experiment of the mice spleen cells in vitro, research it effect on immune function.(4) Study on toxicity test of Huangqi Jingye San: After a drug toxicity the animals reaction is observed and its LD50 is determined. As the limit of a drug concentration and volume, LD50 can not be measured, a maximum tolerance determination of the drug has been made.Results(1) Study on quality standard of the Stem Leaf of Radix Astragali raw material medicine: this product description: cylindrical stem, upper branches, 50~60 cm long, 0.5~1.5cm in diameter, hard and tough, not easy to broke, the broken fibre is strong, and reveals the powder. Skin is yellow-green and wood department is light yellow, texture and radial fractures.Leaves shrivel, yellow-green, leaves alternate and leaf blade elliptic or long ovoid, 0.5~1.5 cm long, 0.5~1.0 cm wide, apex obtuse-pointed, sectional shape or with short point, margin entire.The stipule is lanceolate or triangular. It likes a paper, and it is fragile. Micro smell and slightly bitter taste, chewing the micro has bean smell. Identification, stem cross-sections, leaf crosssections, the spiral duct, fibre and calcium oxalate crystal cluster of this powder is numerous,visible prism.Stem cross-sections: epidermal cells is square, the differentiation of cortex and marrow is not obvious, cortex consists collenchyma with parenchyma.Vascular bundle in the vascular cylinder arrangement is obvious. Leaf cross-sections: Upper and lower epidermis cells are square, the outermost layer of cuticle.The upper layer of palisade tissue and through the main vein. Spongy tissue cells show class circular, calcium oxalate crystal clusters, outside the main vein vascular bundle tenacity, sheath fibers are arranged around the column, it is a ring.Parenchyma cells contain calcium oxalate crystal edges. Wood parenchyma cells contain the occasional calcium oxalate crystal clusters. The spots of TLC were round and clear with good repeatability. The moisture contents were 6.24%~12.24%; the total ash were 8.21%~10.55%;the water-solubility extracts were 12.12%~27.30% and alcohol-solubility extracts were6.89%~10.28%; the total contents of astragaloside were 23.74~26.52 mg·g-1 and astragalus polysaccharide were 23.31~45.70 mg·g-1; the astragaloside IV contents were 0.047%~0.18%;the calycosin-7-O-?-D-glucoside were 0.21%~0.26%.(2) Study on the pharmaceutical research of Huangqi Jingye San: Useing the pharmacopoeia method, the quality standard of Huangqi Jingye San was resolved by the single screening method, through the sieve powder weight 5 is96%~99%. Exterior colour and lustre is uniform, no decorative pattern, color spot. The moisture contents of Huangqi Jingye San were 6.04%~9.28%. Filling quantity differences are not beyond the load limit. According to the market after the packing, the accelerated test and long-term test were relatively stable.(3) Study on the pharmacodynamics research of Huangqi Jingye San:The Minimum Inhibitory Concentration of aqueous extracts of Huangqi Jingye San(annual) on staphylococcus aureus, escherichia coli, bacillus subtilis, pseudomonas aeruginosas was 15.6,62.5, 31.2, 62.5 mg·mL-1. The perennial was 15.6, 31.2, 31.2, 62.5 mg·mL-1. Alcohol extracts of Huangqi Jingye San(annual) were 7.81, 31.2, 31.2, 62.5 mg·mL-1 and the perennial were 7.81,31.2, 15.6, 62.5 mg·mL-1. The Minimum Bactericidal Concentration extracts of Huangqi Jingye San on staphylococcus aureus, bacillus subtilis were 62.5, 125 mg·mL-1 and was respectively31.2, 62.5mg·mL-1 on escherichia coli, pseudomonas aeruginosa. The extracts of Huangqi Jingye San in mice spleen cells in vitro proliferation effect experiment by ConA the high dosegroup has a significant role in promoting, but the middle and the low dose group is on the other hand. The high and middle dose group has a significant role in promoting, and the low dose group is on the other side but excpet aqueous extracts of Huangqi Jingye San(annual) and alcohol extracts of Huangqi Jingye San(perennial) by LPS.(4) Study on toxicity test of Huangqi Jingye San: As the toxicity of the tested drug is low, it is unable to determine its LD50. Within 7days after the treatment, mices' activities and respiratory conditions are normal, and no death.Conclusions(1) Study on quality standard of the Stem Leaf of Radix Astragali raw material medicine: The methods are convenient, fast and repeatable, and the results are accurate and reliable, which can be used to control the quality of the Stem Leaf of Radix Astragali effectively.(2) Study on the pharmaceutical research of Huangqi Jingye San: The methods including particle size, appearance evenness, content determination, moisture content and the load difference are efficacious. Through accelerated test and long-term test, the product character, content, particle size and appearance evenness were within the rules.(3) Study on the pharmacodynamics research of Huangqi Jingye San: The extracts of Huangqi Jingye San on staphylococcus aureus, escherichia coli, bacillus subtilis, pseudomonas aeruginosa have the bacteriostatic effect of highly sensitive and obvious bacteriostatic action. It also can promote the spleen lymphocyte proliferation induced by ConA and LPS in vitro. It prove that Huangqi Jingye San has the promoted function of immune regulation.(4) Study on toxicity test of Huangqi Jingye San: As a result,it is a safety and green veterinary drug products in the low toxicity.