Diversity Of Bacterial And Fungal Communities In The Nasal Cavity Of Xiang Pig

Aims: Theory and practice of micro-ecology is driving people to understand the respiratory tract microflora. Nasal mucosal surface of the upper respiratory tract and other mucosal tissues with the outside world, planting the relatively stable structure and composing of very complex microbial flora. Due to the organizational structure, functions and the impact of external factors, many physiological form is different from other parts of the respiratory tract in the unique ecological environment.Methods and results: Through PCR-DGGE and Real-time PCR method combined with image analysis software Quantity one, DGGE profiles and UPGMA cluster were analyzed, and calculate the diversity index(Shannon index, evenness index, richness index and dominance index). DNA was extracted from the nasal mucosa more objectively reflect the real situation of the diversity of microbial colonization by DNA enzyme binding CTAB method; Pigs get the nasal flora of PCR-DGGE fingerprinting atlas reflects the dynamic changes in the nasal cavity microflora, different growth stages of pigs nasal fungal, bacterial flora structural stability was not significantly different(P> 0.05), Meanwhile fungal and bacterial community structure in the infected and healthy pigs nasal group was also not significant(P> 0.05).; Internal colonization in the peak of the two-month-old pig nasal mucosa, bacterial diversity index was significantly higher than other months, when the stage of May, the lowest nasal microbial diversity index;Conclusion: DGGE profiles of bands clone sequencing detected a large number of uncultured bacteria and fungi, but also to detect common condition of pathogenic microorganisms: Streptococcus pneumoniae, Staphylococcus pyogenes, Staphylococcus aureus, Moraxella sp., Haemophilus influenza, H. parasuis, Candida albicans.; Cloning and sequencing results microorganisms mainly attributable to:Ascomycota, Pezizomycotina, saccharomycotina, Bacteroides, Firmicutes, Actinobacteria. Infection in pigs within the nasal cavity, Staphylococcus aureus is the relative copy number was significantly increased, as the nasal cavity respiratory tract infection pathogenic indicator bacteria.Significance and Impact of Study: Through the diversity of fungi and bacteria of community structure in the nasal cavity pigs, dynamics, and stability analysis, screening habitats cause respiratory infections in pigs pathogenic indicator organisms for the diagnosis and prevention of respiratory disease in pigs provide theoretical reference.