Salt-tolerant Ability And Molecular Identification Of Dense-branches Tamarix

The dense-branches Tamarix represented by Tamarix chinensis ‘Binhaicui' are not only resistant to salinity,drought and wet,but also have no flowers,which avoids them becoming biological invaders.So dense-branches Tamarix were selected as the main experimental materials and analyzed from 4 aspects which are salt tolerance indexes,growth indexes,physiological-biochemical indexes,andor photosynthetic-fluorometric parameters.Besides,their molecular identification cards were established.The results were as follows:Salt tolerance indexes of Tamarix were becoming stationary after 10 weeks in the salt water culture experiment.Correlation between salt tolerance indexes and salt concentration were significant negative(P<0.01).The following results can be obtained after fitting data of salt tolerance indexes.Half-life salt concentration is 3.3% and limiting salt concentration is 4.4%.All the indexes reflected a common trend that with the increase of salts concentration the injuries of Tamarix got worse.And the salt concentration of 3.2%~3.5% can be used a reference value to compare salt-tolerant ability of dense-branches Tamarix with other salt-tolerant plants.In the salt soil culture experiment,the salt tolerance indexes of Tamarix were becoming stationary after 5 weeks.With the increase of salts concentration the injuries of Tamarix got worse visibly.Half-life salt concentration is 1.1% and limiting salt concentration is 1.5%.Cuttage method does not affect their salt-tolerant abilities.In the molecular marker experiment,we have found dense-branches Tamarix DNA extraction methods and PCR amplification conditions.There are 9 pairs of primers were selected to serve for molecular identification cards.It can explain genetic relationship within the group and prove genetic diversity of dense-branches Tamarix at the molecular level.It also distinguishes dense-branches Tamarix from other Tamarix and lays the foundation for intensive study.