| Escherichia coil was isolated from fresh manure samples that were collected from the farms in Kelamayi、Changji、Hutubi and Manasi in Xinjiang in spring and autumn. Broth dilution method was used to test the drug resistance of isolated E. coli. After obtaining the drug-resistance strains, PCR was used to test the b-lactamase and 16 S r RNA methylase. The characteristics and popular features associated with the drug-resistance genes from different regions and seasons were then compared. The results were as follows: 1. The b-lactamase enzymes and aminoglycosides antimicrobial agents of E. coli obtained from different regions in Xinjiang(1) The drug-resistance rates of swine E. coli in Kelamayi to Amoxicillia/ Clavulanate、Ampicillin and Gentamicin were >50%(i.e.75.48%, 64.29% and 64.22%, respectively).(2) The drug-resistance rates of swine E. coli in Changji to Amoxicillia/ Clavulanate、Ampicillin and Gentamicin and Apramycin were >50%(i.e. 90.20%, 87.36%, 75.38 and 55.12%, respectively).(3) The drug-resistance rates of swine E. coli in Hutubi to Amoxicillia/ Clavulanate、Ampicillin and Gentamicin were >50%(i.e. 93.44%, 84.90% and 79.65%, respectively).(4) The drug-resistance rates of swine E. coli in Manasi to Amoxicillia/ Clavulanate、Ampicillin and Gentamicin were >50%(i.e. 84.93%, 76.86% and 72.93%, respectively). The resistance of the multi-drug resistant strains from the regions listed above ranged from 0-6. Those isolated from Changji was mostly 4 resistance, while the multi-drug resistant strains from the other regions was mostly 3resistance. The most serious drug-resistance condition was the swine bacteria from Hutiubi, followed by Changji、Manasi and Kelamayi. 2. The resistance results of β-Lactams and Aminogly cosides for Swine E. coli obtained from Xinjiang in spring and autumn(1) The resistance rates of Swine E. coli obtained during spring to Amoxicillia/ Clavulanate、Ampicillin and Gentamicin were >50%(i.e. 83.47%, 82.38% and 74.15%, respectively). The resistance rates of Swine E. coli obtained during autumn to Ampicillin、Amoxicillia/ Clavulanate、Ampicillin and Gentamicin were >50%(i.e. 81.68%, 66.86% and 62.52%, respectively). The resistance of the multi-drug resistant strains from different seasons ranged from 0-6. Those isolated in spring was mostly 3 resistance, while those isolated in autumn was mostly 4 resistance. The resistance rate of wine E. coli obtained during spring was higher than those obtained during autumn. 3. Comparison of β-lactamase and 16 S rRNA Methylase genes of resistant E. coli obtained from different regions of Xinjiang(1) The detection rate of blaTEM gene was 100% in the isolated Swine E. coli from different regions of Xinjiang. In addition, blaOXA and blaCMY-2 were also detected in the isolated Swine E. coli from different regions of Xinjiang.(2) rmtB gene was not detected in Kelamayi; blaLAP-1 gene was not detected in Hutubi.(3) blaSHV gene was detected in Kelamayi and Changji;armA gene was detected in Hutubi. ⑷ blaKPC gene was not detected in any of the isolated Swine E. coli. Other than blaTEM gene,the detection rate of other genes in descending order was Manasi, Hutubi, Changji and Kelamayi. 4. Comparison of β-lactamase and 16 S rRNA Methylase genes of resistant E. coli obtained during spring and autumn(1) blaTEM gene was detected in Swine resistant E. coli obtained in spring and autumn; the detection rate was 100%. In addition, blaOXA, blaCMY-2, blaLap-1 and rmtB genes were also detected.(2) blaSHV and armA genes was detected in swine resistant E. coli obtained during autumn.(3) none of the strains was reported to have blaKPC gene. Other than blaTEM gene, the detection rate of other genes was higher for those obtained during autumn than spring.The drug resistance of swine E. coli obtained during spring and autumn as well as different regions of Xinjiang to Amoxicillia/ Clavulanate、Ampicillin and Gentamicin was serious. Moreover, blaTEM gene was the predominant gene and there was a co-existence of multiple drug resistance genes. |
