Effects Of Feeding Phytoestrogens On Lactation Performance, Contents Of Sex Hormone In Milk And Plasma,And Equol Of Dairy Cows

This paper was conducted to investigate the effect of fed dainzein, formononetin and composition on content of equol in milk of dairy cows in the diet, and contents of sex hormone in milk and plasma of dairy cows.A total of 40 Holstein cows were selected and randomly divided into 4 groups, by the health, age and lactation stage. The control group was fed TMR, and the other in 3 trial groups were fed TMR supplementer with 2.5 g dainzein(trial group ?), 35 g formononetin(trial group ?), 1.25 g dainzein and 17.5 g formononetin(trial group ?), respectively. The total duration of the trial was 127 days, the first 7 days of the trial was the preliminary period and the formal trial lasted for 120 days. The milk and plasma samples were collected in the trial of 0, 30, 60, 90, 120 days, and-20 ? saved for later use, respectively, determination of lactation performance, content of sex hormone in milk and plasma and equol in milk, biochemical index, antioxidant capacity of plasma. The results showed as follows: compared with the control group, the milk yield and milk protein yield in trial group ?, ? was significantly increased(P<0.01); the milk protein rate in trial group ? was significantly increased(P<0.05); the lactose yield in trial group ?, ?, ? was significantly increased(P<0.01); the BUN in trial group ?, ? was significantly increased(P<0.05); the ALB, GLB, TP of blood were no significantly difference(P>0.05); the T-AOC activity of blood in trial group ? was significantly increased(P<0.05); the GSH-Px activity of blood in trial group ?, ? was significantly increased(P<0.05); the CAT, SOD activity of blood in trial groups had a upward trent, but no significant difference(P>0.05); the MDA in trial group ?, ? was significantly decreased(P<0.05); the E2-17? of blood in trial group ?, ? was significantly increased(P<0.05); the P and PRL of blood in trial group ? was significantly increased(P<0.05); the E1, E3, LH, FSH of blood were no significantly difference(P>0.05); the E2-17? of milk in trial group ? was significantly increased(P<0.01); the P of milk in trial group ?, ?, ? was significantly increased(P<0.05); the E1, E3 of milk were no significantly difference(P>0.05); the equol of milk in trial group ?, ?, ? were 166.91?g/L(P<0.01), 517.99 ?g/L(P<0.01), 402.59 ?g/L(P<0.01), respectively. It is concluded that feed phytoestrogens can improve the lactation performance of Holstein cows, when supplementary feeding 2.5 g dainzein was the more obvious effect; feed phytoestrogens can increased plasma antioxidant capacity and content of BUN of cows, the content of ALB, GLB, TP were no significant difference; effect of the content of E2-17?, P, PRL, the content of E1, E3, LH, FSH were no significant difference; effect of the content of E2-17?, P, the content of E1, E3 were no significant difference; the content of equol was significant increased, when supplementary feeding 35 g formononetin was the more obvious effect, and dainzein and formononetin have interaction.