| Plants play an important role in the Se transformation process of natural. It can absorb low-molecular organic or inorganic selenium, and converted to organic selenium compounds. Liang Shan Yi Autonomous Prefecture of Sichuan Province was located in the cold and high-altitude areas. Buckwheat is the main cultivation plants and the stable food of Yi peploe. Liangshan Prefecture buckwheats contained aboundant proteins and flavonoids compounds because of non-pollution, longer growth period, and temperature largely difference between day and night, which are fine varieties of buckwheat in our country. However, Liangshan Prefecture is situated in regions of Se deficient. The amount of Se in soil of these areas is very low, so Se content in buckwheat lower than other areas when the plant cultivate in these soils, and the role of plant Se compounds for human bodys may not work well. Therefore, cultivation buckwheat plant with adding inorganc is effective methods to enhance Se content in buckwheat. The specation of Se in Se-enriched plants is such an important course that attracted lots of scientists? interests. We studied the mains occurring specation of Se in the Se-enriched buckwheats?leaf, stem and seed? to learn the conversion efficiency of Se and distribution of different Se species in Se-enriched buckwheats.Extract Se-binding proteins in seeds and Se-containing compounds in leaves and stems with 75% ethanol, deionized water, 0.5 mol·L-1 NaCl, and 0.1 mol·L-1 NaOH. And determine the contents of protents in Se protent and Se compounds by formaldehyde-acetyl acetone coloration spectrophotometry, the concertration of Se by hydride generation atomic fluorescence spectrometry. It showed that distribution of Se contents in Se-enriched buckwheats with adding two kinds of inorganic Se was leaves>seeds>stems. Siginificant positive correlations was found between protein and Se in seeds?P<0.01?, and positive correlations in leaves?P<0.05?. It demonstrated preliminarily that more than 80% of Se in seeds transforms in the forms of Se-binding proteins, and more than 50% of inorganic selenium is soluble form in leaves and stems. The organic Se conversion efficiency and absorptivity cultivated by Na2SeO4 was higher than Na2SeO3 in Se-enriched buckwheats.Analyze Se specation in Se-enriched buckwheat by HPLC-UV-HG-AFS. This method detection limit was 1.6, 26.1, 19.5, and 10.8 ?g·L-1, respectively. Precision was 1.71%, 2.86%, 5.34%, and 4.16%, respectively. The results showed that about 32.98% to 33.10% of Se was combined with methionine in Se-enriched buckwheat seeds, and about 2.90%-15.68% of Se was specation of Se???. About 1.84% to 11.24% of Se was specation of Se??? in buckwheat leaves. There was no detected Se specation in buckwheat stems. Se compounds in the leaves and stems were the nonprotein-bound Se. The transformation efficiency of inorganic Se to Se-binding proteins was no significant difference between buckwheat plants spraying with selenite and selenate. |
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