The Study On The Drug Resistance Of Type A Influenza Virus In Guangxi Antonomous Region

Via combining the sites analysis of drug resistance of amino acid and the suppression methods of micro-cell disease, this study focused on the drug resistance of 18 strains of H1N1 swine flu virus and an H3N8 equine influenza virus subtype in Guangxi Zhuang Autonomous Region within recent years.After finishing the cloning sequencing and gene sequence alignment of gene NA and M, the results showed that 1. The drug resistance of M2 gene in 18 strains of H1N1 swine flu virus was caused by S3 IN mutation; 2. The drug resistance of H3N8 equine influenza virus caused no mutation. However, among all the 18 strains of H1N1 swine flu virus, in Guangxi Zhuang Autonomous Region within recent years, only the gene GX3 caused E119K mutation, No.6 caused El 19G mutation and gene G2 caused N295D mutation on the enzyme's active site of NA and its relative sites. The rest of the 15 strains of H1N1 flu virus had no mutation. As for the strain of H3N8 equine influenza virus, which caused S247A mutation.During the experiment of suppression methods of micro-cell disease,1 mg/ml of amantadine hydrochloride was used to inhibit the strain of H3H8 equine influenza virus from infecting MDCK cells. This strain was sensitive to the amantadine hydrochloride and the effective concentration is up to 10-1.5. The 18 strains of swine flu H1N1 virus were drug-resistant and all the MDCK cells were infected and caused lesions under the effective concentration of 10-1.5.During the experiment of suppression methods of micro-cell disease,1 mg/ml of oseltamivir phosphate was used to inhibit the 18 strains of swine flu H1N1 virus from infecting MDCK cells. These strain were sensitive to the oseltamivir phosphate and the effective concentration was between 10-1-10-2.76. The H3N8 equine influenza virus were drug-resistant and MDCK cells were infected and caused lesions under the effective concentration of 10-1-10-2.76.