Quinagolide Regulate Lipogenic Genes In Lactational Mammary Gland Of Rats

Prolactin plays an important role in the start-up and maintenance of lactation. Prolactin can promote ductal extension, milk production, and can promote the synthesis of milk components, especially affect the synthesis of milk fat. In order to study the regulatory mechanism of prolactin on milk fat synthesis, this study used dopamine agonists(D2)--quinagolide to inhibit the prolactin release in lactation rats, and used digital gene expression profiling to analyze and compare expression differences of milk fat synthesis genes between the quinagolide and control in breast tissues of lactation rats. In order to verify the accuracy of the sequencing results,this study selected the 10 genes to the measure the expressions by fluorescence quantitative PCR technology.The transcriptome sequencing test for two groups of mixed samples, and received an average of 14458067 clean reads. Two gene library got the 12843(quinagolide) and 13639(control) genes, respectively. Taking the |log2Ratio(Q/C)?2, FDR?0.001| as a standard to compare the genes expression differences between two groups of breast tissues.There are a total of 2823 differentially expressed genes, including 262 down-regulated genes and 2561 up-regulated genes, respectively accounted for 3.9% and 42.3% of the total number of differentially expressed genes. The results in transcription show that:Prolactin increase synthesis of milk fat by promoting differentiation, growth of breast epithelial cell.Prolactin can regulate the gene expression of FABP3, ACC, FADS1, INSIG1.