| Meloidogyne incognita is one kind of important omnivory plant pathogenic nematode with highly specialization, widely distributed around the world, and can parasitic in most higher plants. HeberNem-S wettable powder is a biological nematicide which researched by Center of Genetic Engineering and Biotechnology in Camaguey, Cuba, and the active ingredient is Tsukamurella paurometabola C-924 which isolated from soil. In this thesis, we had studied the indoor virulence of 3×1012 CFU/g HeberNem-S WP to Meloidogyne incognita J2, the efficiency of HeberNem-S to root knot nematodes in pot experiment and field experiment. We had detected the colonization of Tsukamurella paurometabola C-924 in soil, and the influence on population of bacteria, fungi and actinomycetes in the soil, the antagonism between several common soil borne diseases. The results would be a scientific basis for its utlization in China. The main results are as follows:1. The dipping method was used to study the lethal effect of HeberNem-S to M. incognita J2. When at the concentration of 1.5×1011,1.5×109,1.5×107CFU/L, the correction mortality rates of J2 after 24 h were 90.64%,76.99% and 90.64% respectively. The correction mortality rates of J2 after 48 h were 92.93%,80.83% and 71.60% respectively. When at the concentration of 1.5×105?1.5x109, there were no difference between the correction mortality rates on J2. When the M. incognita J2 was death caused by HeberNem-S, they often are rigidity or bending.2. The safety of HeberNem-S WP to tomato seedlings in pot experiment in greenhouse, the influence on several growth parameters of tomato and its efficacy to root-knot nematode were investigated. When at the dosage of qx,2x,4x to the highest registered dosage, they were safe to three varieties of tomato seedlings. The efficacy of 2.0,2.5,3.0 kg/ha were 55.56 %,57.14%,65.71% respectively 30 days after transplanting. The efficiency were 35.48%, 46.77%and,46.77% 60 days after transplanting. The efficacies of 2.5 kg/ha were higher than 2.0 kg/ha, and the difference between HeberNem-S WP 3.0 kg/ha and 4% abamectin CS 7.5 kg/ha were small. HeberNem-S showed significant role in promoting the height and stem diameter of tomato plant at the beginning of the trial.3. Collecting the soil in pot experiment to test the number changed of T. paurometabola C-924 in soil, and test the number changed of bacteria, fungi and actinomycetes when treatmented with 3×1012CFU/g HeberNem-S WP 2.5 kg/ha. The number of T. paurometabola C-924 in soil were reduced gradually under the three dosages treatment as the increase of application days, the decline were increased 14 days after application; The number of bacteria, fungi showed a slow decline after treatment, and the downward trend of fungi is greater than bacteria, the actinomycetes number dcreased rapidly after 1 day after transplant, and downward after it.By studying the antagonism of Tsukamurella paurometabola C-924 to seven soil-borne diseases, the results showed that, the antagonism effects of T. paurometabola C-924 was highest to Sclerotium rolfsii, the inhibitory rate is 31.10%, and higher than the control strain Paenibacillus polymyxa to this strain (29.65%), followed by Fusarium axysporum and Aspergillus niger, the inhibition rate were 29.23%,23.57% respectively.4. Using drip irrigation system to study the control effect of 3 × 1012 CFU/g HeberNem-S WP 2.5 kg/ha to tomato root-knot nematode. The galling index was 25.41 after treatment, and the control effect was 38.65%, less than the control pesticide of 4% abamectin CS 7.5 kg/ha (48.34%) but the difference was not significant. The number of M. incognita J2 was 109.27 in average gram of the root, higher than the control pesticide of 4% abamectin CS 7.5 kg/ha (76.51), in addition, HeberNem-S was also reduced the number of J2 in soil. |
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