Effects Of Soil Conditions On Yield And Quality Of Different Strain Salvia Miltiorrhiza Bge

Salvia miltiorrhiza(S. miltiorrhiza) Bunge is broadly used as Chinese medicine for the clinical treatments of cardiovascular and cerebrovascular diseases, chronic renal failure, neurasthenic insomnia, dysmenorrheal, and liver cirrhosis. The chemical components of S. miltiorrhiza were complex. Previous studies had demonstrated S. miltiorrhiza species show variations in the contents of bioactive compounds according to various environmental conditions and different strains. The quality variations inevitable affected its pharmacological activities, and even posed a threat to the safty and health of people. This article showed the effect of different planting areas and strains on yield and accumulation of bioactive constituents in S. miltiorrhiza, and provided a theoretical basis for people to select the optimal cultivar and the optimal environmental condition.Four different S. miltiorrhiza strains were selected as experimental materials, and were planted in Zhuyang, Changqing, and Taian. We sustematically described the metabolic profiles of S. miltiorrhiza using LC-MS in conjuction with multivariate statistical analyses, aimed at monitoring their biological variations of secondary metabolites related to three planting areas and four S. miltiorrhiza strains. At the same time, we studied the dunamic accumulation of dry matter and root dry matter based on the days after transplanting. The enzyme activities of PAL, 4CL, CAD, and TAL were also determined. The main results were as followed:1. Finally, in total, 40 out of the 66 metabolites, including 16 phenolic acids, 20 tanshinones and four other conpounds(i.e. luteolin, royleanone-4, 7α-hydroxyallyl-royleanone, and β-sitosterol) could be putatively identified in S. miltiorrhiza by using LC-MS.2. Effect of planting areas on yield and qualityPlanting areas significantly affected the yield and the content of bioactive compositions in S. miltiorrhiza. In conclusion, S. miltiorrhiza strains grown in Zhuyang had lower yield and higher phenolic acids. S. miltiorrhiza strains grown in Taian had higher yield and lower active constituent content and enzyme activities. Changqing location had relatively higher bio-yields of phenolic acid and tanshinone, which made it suitable for S. miltiorrhiza cultivation. Furthermore, by using OPLS-DA, 16 secondary metabolites could be used as potential location-specific makers in S. miltiorrhiza.3. The difference on yield and quality in different starin S. miltiorrhizaStrain significantly affected the yield and the quality in S. miltiorrhiza. ZH105 strain could maintain relative salvianolic acid B stability at different environment conditions, indicating that ZH105 strain was possibly related to resistances. By using OPLS-DA, 14 secondary metabolites could be used as potential strain-specific makers in S. miltiorrhiza.4. The dominant factor for the variation of secondary metabolites in S. miltiorrhiza.Both planting area and strain affected the content of bioactive compositions in S. miltiorrhiza. The dominant factor for the variation of secondary metabolites was subject to change. Our research showed that the dominant factor could be planting area or strain. Therefore, this article laid a good foundation for quality control of traditional Chinese medicine.