Germplasm Innovation Evaluation And Artificial Cultivation Study Of Dendrobium Officinale

This research through the 12 different origin of Dendrobium officinale fingerprint establishment, Dendrobium officinale polysaccharides, amino acids, trace elements, heavy metal content determination, Dendrobium officinale germplasm resources evaluation; At the same time through the Dendrobium officinale polyploid, tissue culture, cultivation matrix, heat resistance and so on production innovation research, in order to supply high quality Dendrobium officinale medicinal materials theory and practice basis. The specific contents and research results are as follows:1.With different origin of Dendrobium officinale fresh stem produced froml2different areas segments as material, The results show; that the established different producing area features a total of 23, similarity was 0.918-0.987, shows different origin of Dendrobium officinale have differences, but there was no difference in the chemical composition and chemical content and clustering analysis will be 12 different origin of Dendrobium officinale is divided into four categories:From yu xi、ning hua、nan jing、tian tai Cu, As, Cd content exceed 26.25%,44.5%,27.33% and 44.5%, respectively, other sources of Cu, As, Cd, Hg,Pb of heavy metal content.2.Through the indentification of variant plants about mutant cells in morphology, cytology, and the Identification, in order to obtain steady tetraploid plants and analyzes its physiological characteristics. we can found the relative DNA content of tetraploid was 400, diploid was only 200 by flow cytometry analysis to determine their ploidy. Tetraploid plants leaf chlorophyll content, soluble protein, soluble sugar content were higher than that of diploid, were 5.03 mg·g-1,3.59 mg·g-1,2.98 mg·g-1; The main antioxidant enzymes in the leaves of POD and SOD activity were significantly higher than that of diploid, Were 9.08 mg-1·min-1,180.4 U·mg-1, and the tetraploid plants significantly reduces the accumulation of MDA content.3. The experimental results:(1) step into seedlings:Dendrobium officinale from the beginning of the seed germination, proliferation without proliferation differentiation of protocorm and stem section directly into seedling; (2) the Dendrobium officinale for seed germination:MS+6-BA2.0 mg·L-1+NAA0.5 mg·L-1+KT1.0 mg·L-1+mashed potatoes 150～200 g·L-1, the optimal culture medium for strong seedling root:1/2MS 6-BA 2.0 mg·L-1+NAA0.3mg·L-1+mashedpotatoes150～200g·L-1 banana puree+150～200 g·L-1; (3) sheet of Dendrobium officinale a hormone-free tissue culture method, ranging from Dendrobium officinale disinfected seed powder, fruit powder seed germination medium by cultivation, round bulb of proliferation differentiation, stem segments and stem section of root seedling four different stages of growth and development; (4) proliferation in the process of the propagation of Dendrobium officinale section made of natural additives (potatoes, bananas).4. In the same cultivation management conditions, transplanting 30, tissue culture seedlings in the treatment of GC, GD survival rate of 97.3%,95.8%, significantly higher than other processing and control, which deal with GC of plant height, stem diameter, root length and the new root number also significantly higher than other processing; Transplanting 365 d, deal with GC survival rate, plant height, stem diameter, root length and the new root number is still the highest, reached 94.6%,7.87 cm,0.307 cm,5.57 cm, 7.67 and other processing significant difference, Dendrobium officinale polysaccharides and total alkaloid content in the treatment of GC in the maximum at the same time, were 8.88% and 0.30% respectively and other processing significant difference and contrast.5 The effects of different concentrations of salicylic acid on the heat induction effect. when concentration of SA for 0.5～1.5 mmol·L-1, the activity of CAT and APX in leaf was significantly improved; The concentration of SA for 1.5～2 mmol·L-1 can be effectively, inhibit the increase in the MDA, increase Pro, soluble sugar and soluble protein accumulation,there will be the significant differences between the different concentration1.5 mmol·L-1 concentrations can reach the best treatment effect and provide a favorable basis for the facility cultivation of Dendrobium officinale.