The Role Of Autophagy In Fermentation Efficient And Pathogen-antagonist Of Saccharomyces Cerevisiae

Autophagy is a physiological activity of organisms in adversity stress, Saccharomyces Cerevisiae is one of the antagonism yeast, the research of it an important part of the research in the biological control.Because the S.cerevisiae application in biological control has many advantages, but the antagonism process will be stressed by ethanol, and Reactive Oxygen Species(Reactive Oxygen Species, ROS) poisoning, but under the condition of autophagy in ethanol stress effect on S.cerevisiae antagonism pathogen is not yet known, ROS on the role of autophagy not settled yet.In order to study the autophagy in S.cerevisiae antagonistic postharvest fruits and vegetables' pathogens, and the role of autophagy under ethanol stress in S.cerevisiae, we chose Colletotrichum gloeosporioides, Botrytis cinerea Pers, Rhizopus stolonifer, Alternaria solani as the main object of S.cerevisiae antagonism experiment to sift the one can be inhibited the growth of pathogenic bacteria by S.cerevisiae, and the autophagy gene mutations use the tablet confrontation to determine the role of autophagy in antagonism process. Use the ROS scavenger(GSH, NAC), antioxidant enzyme inhibitors(2-ME, 3-AT), respiratory chain inhibitors(Antimycin A, Rotenone), autophagy inducers(Rapamycin),autophagy inhibitor(3-MA) to dispose S.cerevisiae and its autophagy gene mutations, testing the glucose consumption ability of wine, the distribution of the intracellular ROS, autophagy genes expression.The results show that S.cerevisiae has no effect on Rhizopus stolonifer, but have the antagonist effective onColletotrichum gloeosporioides, Botrytis cinerea Pers, Alternaria solani, and this effect has concentration dependence,:the higher the concentration, the stronger the antagonistic is. Macroautophagy gene mutations atg1 ? and atg8 ? can result in weak antagonism, mitophagy gene mutations atg11 ? and atg32 ? can result in stronger antagonism.The ethanol stress can result in S.cerevisiae generates ROS, induced autophagy and mitophagy.Macroautophagy and mitophagy are necessary for S.cerevisiae fermentation, the lack of related gene results in the decrease of S.cerevisiae fermentation efficiency.Under the ethanol stress, macroautophagy and mitophagy,ROS are induced, or ROS is mediated by ethanol stress caused by macro autophagy and mitophagy.Under ethanol stress, conditions of O2·- and H2O2 and O2·-in the mitochondria, the increase of H2O2 in the cytoplasm resulting in a decline in S.cerevisiae fermentation efficiency and increased mortality,macroautophagy and mitophagy are induced. Rapamycin can increase ethanol stress autophagy and mitochondrial autophagy occurs; under the condition of ethanol stress S.cerevisiae in type ii mitophagy(independent of PI3K), 3- MA can not inhibiti the mitophagy.Through the above results indicate that:(1) can be induced by the macro autophagy and the inhibition of the mitophagy strengthen S.cerevisiae on postharvest pathogens antagonistic, which is applied in production practice;(2) can inhibit the macro autophagy and inhibition of mitophagy, enhance the resistance to ethanol stress of S.cerevisiae, improve the efficiency of the fermentation and the survival rate, which is applied in production practice.