| Biomass energy is one of the earliest sources of energy used in human society,which has the advantages of wide distribution,renewable and low cost.At this stage,biomass energy accounts for about 10 % of the world’s energy supply,and has considerable prospects.The development and utilization of biomass energy is of great practical significance to the sustainable development,the protection of national energy,the improvement of social environment and the reduction of greenhouse gas emissions.In view of this phenomenon,the common biomass waste in Chongqing area(including the municiple sludge,rotten citrus,kitchen waste,Chinese medicine residue,distillers’ grains,garden waste,pig manure and cattle manure)was selected as raw materials for anaerobic digestion,using a separate anaerobic digestion test analysis of different kinds of raw material waste anaerobic digestion potential.The biomass waste which has better biogas production potential was selected as materials for anaerobic co-digestion test to investigate the optimal ratio of different kinds of biomass waste,providing a theoretical basis for the comprehensive utilization of biomass waste gas.In addition,three dimensional fluorescence and microbial population analysis(16S rDNA)were used to analyze the organics,population structure and diversity of the digestive system.The study on the potential of methane production from the municiple sludge shows that the potential of gas production in the western part of Chongqing is generally better than that in the eastern part of Chongqing.Although the Wanzhou district belongs to the eastern region of Chongqing,but because of its good development,large population,resulting in the sludge production is huge,so the biogas production potential was significantly higher than that of the surrounding area;rotten citrus,kitchen waste,Chinese medicine residue and distillers’ grains is not suitable for separate anaerobic digestion,because pH value will decreased significantly in the digestive process,and it will seriously affect the methane production,resulting in the methane production was less than 50 m L;pig manure,cattle manure and garden waste has a productive effect in separate anaerobic digestion,methane production was 340 m L,261 m L and 265 m L respectively,but ammonia nitrogen of the digestive juice of pig manure in high concentration may affect subsequent processing of digestion products,and it will reduce the utility of the separate anaerobic digestion.When the kitchen waste was anaerobic co-digestived with garden waste,the methane production was increased by the ratio of 1:3,1:4 and 1:5,and by the ratio of 1:3 to enhance the highest rate reached 62.03%,the methane production was 352 m L;and the rotten citrus was anaerobic co-digestived with garden waste,the system of methane production increased when the ratio of 1:4 and 1:5,and by the ratio of 1:4 to enhance the highest rate reached 53.25%,the methane production was 341 m L;when the distillers’ grains was anaerobic co-digestived with garden waste,methane production of all proportion decreased,illustrate that is not suitable for the distillers’ grains improving the production of methane by anaerobic co-digestion with garden waste,when the ratio was 1:4,it has the lowest rate of decrease,-13.88%,the production of methane is 195 m L;kitchen waste was anaerobic co-digestived with pig manure,when the ratio of 1:4 and 1:5,production of methane was improved,when the ratio was 1:4,the highest rate of increase was 56.02%,and the methane production was 448 mL.There were some differences in the population structure of methanogenic bacteria in the separate anaerobic digestion by kitchen waste or garden waste.The dominant genus of methanogenic bacteria in the separate digestion of kitchen waste is Methanospirillum,Methanobrevibacter,Methanobacterium and Methanoculleus;the dominant genus of methanogenic bacteria in the separate digestion of garden waste is Methanosarcina,Methanobacterium and Methanolinea;the dominant genus of methanogenic bacteria in the co-digestion of kitchen waste and garden waste by the ratio of 1:3 is Methanobacterium,Methanosarcina and Methanospirillum;the dominant genus of methanogenic bacteria in the co-digestion of kitchen waste and garden waste by the ratio of 1:4 is Methanobacterium,Methanosarcina and Methanolinea. |