Lable-free Electrochemical Immunosensor For Some Phenols

Electrochemical immunosensor which is based on theimmunoreaction possesses good sensitivity,selectivity and needs no complicated instruments.Lable-free electrochemical immunosensor have been developed due to no need of complicated antibody or antigen labeling procedures,and the interaction between antibody and antigen can be directly monitored.In this paper,Lable-free amperometric immunosensors were built for bisphenol A or salbutamol detection.Chapter 1 This chapter gave an overview of traditional immunoassay and immunosensor.The classification and detection principle of electrochemical immunosensor,especially the label-free amperometric immunosensor were described.The application of nano-materials in the development of electrochemical immunosensor were introduced.And the researching meanings and main contents of this thesis were summarized.Chapter 2 In this chapter,a label-free electrochemical immunosensor for sensitive detection of bisphenol A was reported.MWCNTs and gold nanopartical(AuNPs)were modified on glassy carbon electrode surface to enhance current response and immobilize the Anti-BPA,and rutin was used as the redox probe.The peak current change of rutin collected by linear sweep voltammetry(LSV)due to the specific immuno-interaction on the modified electrode surface was utilized to detect bisphenol A.The detection range of BPA was 1 X 10-8～1×X 10-6 M with the limit detection of 8.7×10-9 M(3 σ/k).And it was finally applied to BPA determination in the sample solution of fresh-keeping film.Chapter 3 A lable-free electrochemical immunosensor for bisphenol A was built in this part.Acetylene black-chitosan-gold composite(AB-CS-Au)was prepared as a signal amplifier and anti-BPA was immobilized at modified electrode through AuNPs,chlorogenic acid(CGA)was used as the redox probe.the relationship between BPA concentration and current response collected by differential pulse voltammetry(DPV)was used to quantitative analysis of bisphenol A.The detection range of BPA was 7.5 X 10-9～1.0 ×10-6 M with the limit detection of 6.4 × 10-9 M(3 σ/k).Finally,it was applied to BPA analysis in the sample solution of mineral water bottle.Chapter 4 A competitive lable-free immunosensor for salbutamol detection was fabricated.The multiwalled carbon nanotubes-platinum-nafion(MWCNTS-Pt-Nafion)composite was prepared as signal amplifier,anti-SAL was immobilized at modified electrode through AuNPs and the acetyl aminophenol was used as the redox probe.After salbutamol-bovine serum albumin(SAL-BSA)competitively binded the antibody at modified electrode with salbutamol,the current response of acetyl aminophenol on the modified electrode collected by square wave sweep voltammetry(SWV)was used to quantitative analysis of salbutamol.The detection range of salbutamol was 1.0 × 10-9～1.0 × 1 0-7 M,with the limit detection of 3.32× 10-10 M(3 σ/k).It was finally applied to the detection of salbutamol in the sample solution of pork.