Experimental Study On Bone Tissue Biological Response Effect Of A New Type Of Alumina Ceramic

Objective:In this study,we retrospectively analyzed the application of microcrystalline ceramic artificial joints,showing excellent properties of ceramic materials and satisfactory long-term effects.However,in the long-term follow-up study,we found that there are deficiencies.Based on this consideration,we will develop a new type of alumina-based composite ceramic material in this study and apply hydroxyapatite coating on the ceramic surface to achieve bone growth.The bone bioactivity of the complexes was studied by cytological experiments.Methods:1.Through the database of the First Affiliated Hospital of Nanchang University,19 cases of microcrystalline total ceramic arthroplasty were followed up from 1979 to 1980.Due to the limitations of existing data,changes in place of residence,and death of the case,only 10 cases(11 hips)were followed up.The average follow-up period was 36 years(35-37 years).Harris scores were recorded,and image analysis was performed.2.A new alumina-based composite ceramic material was prepared by in situ hydrolysis,nano-ZrO2 coating,hot isostatic pressing and other technical methods.Titanium alloys were used for comparison.The surface morphology and chemical composition of the materials were evaluated by scanning electron microscopy(SEM)and energy spectrum analysis.3.The MC313-E1 cells were co-cultured with the material and the following methods were used to evaluate the in vitro bone bioactivity of the material: CCK-8 colorimetry,DAPI staining,laser confocal scanning microscopy,and SEM were used to study cell adhesion,proliferation,and Spreading capacity.Results:1.No obvious osteolysis was found around the ceramic prosthesis.There were 2 fractures and 2 revisions.One case had a history of impact but no fragmentation.The final assessment of the Harris score averaged 75 points.2.The scanning electron microscopy shows that the new alumina-based composite ceramics has a layer of apatite on the surface of the titanium alloy.3.Compared with titanium alloys,the new alumina-based composite ceramics can promote the adhesion,proliferation,spreading and osteogenic differentiation of MC3T3-E1 cells.Conclusion:The microcrystalline ceramic joint prosthesis showed no obvious wear and no osteolysis around the prosthesis during follow-up,showing good biocompatibility.The new alumina-based composite ceramics modified by in-situ hydrolysis,nano-ZrO2 coating,hot isostatic pressing and the like have good biological activity,and can effectively promote the adhesion,proliferation and spreading of osteoblasts,and strengthen bones.The osseointegration of the implant/interface is expected to be used clinically as a new type of intraosseous implant material.