The Process Optimization Of Maotai Liquor Fortified High Temperature Daqu And Its Comparative Research

In this paper,10 strains of Bacillus were screened from Fumao high temperature Daqu,and were re-screened on the basis of enzyme activity score and aroma score.Four strains with high activity of enzymatic enzyme and high flavor were selected as the functional bacteria Which used in the production of high temperature Daqu,and use the response surface analysis method to optimize the main process parameters such as the amount of water,the temperature of the grain and the amount of the functional bacteria and so on.The optimum parameters of the process are:the water added to grain is 39.19%,the temperature of the grain is 32.92 ℃,and the dosage of the functional bacteria is 1.09%.The changes of 6 kinds of enzyme activity(amylase、glucoamylase、protease、cellulase、lipase、pectinase)、water content、starch content、acidity and temperature during the fermentation process were analyzed.Real-time PCR technology was used to analyze the amount changes of total bacteria and Fortified functional bacteria during the fermentation process of Fortified high temperature Daqu and ordinary high temperature Daqu,and analyze the amount changes of total bacteria、yeast and Fortified functional bacteria during the fermentation of accumulated fermented grains and fermented grains.High-throughput sequencing technology was used to analyze the bacterial and fugal flora structure of high temperature Daqu and ordinary high temperature Daqu,and two-dimensional electrophoresis was used to study the proteomics.Physicochemical index was tested and the results showed that there were no significant differences in water content,starch content and saccharification rate between the two kinds of high temperature Daqu,but the acidity and fermentation power were quite different.The results showed that there were 21 kinds of flavors in two kinds of high temperature Daqu by using HS-SPME-GC-MS technology to analyse the aroma components of Fortified high temperature Daqu and ordinary high temperature Daqu.Pyrazine Compounds,benzaldehyde,methyl benzoate,furfuryl alcohol,benzyl alcohol,phenylethanol in Fortified high temperature Daqu was significantly higher than the ordinary high temperature Daqu.Using the fortified high temperature Daqu in brewingMaotai liquor,is conducive to improving the quality of Maotai liquor.