Synthesis Of Multifunctional Nanomaterials And Its Application In Electrohemiluminescence Immunoassay For β-adrenergic Agonist

The unique catalytic property of metal nanoparticles can amplify the signal with the excitation of themselves or another metal nanoparticle.It is also possible to amplify the signal by encapsulating a large number of molecules into the nanomaterials.Hence,multifunctional nanomaterials are widely used to assemble new types of biosensors.In this paper,CdSe@SiO₂,PAMAM-SNR and AuNPs were synthesized and used in electrochemiluminescent immunoassay（ECLIA）.The dissertation includes four chapters:In chapter 1,it expounds the background and significance of the research.This paper introduced electrochemiluminescence,immunoassay and the applications of multifunctional nanomaterials in ECLIA and sensor.In chapter 2,a multiple signal amplification strategy for ECL immunosensor was designed based on AuNPs,PAMAM and SNR.The excellent electric conductivity made AuNPs-PAMAM-SNR on the surface of GCE act as good accelerators for improving the electrochemical reaction efficiency of QDs and K₂S₂O₈.In addition,PAMAM functionalized SNR as probe with numerous amino groups could be employed to bond abundant actived QDs to further amplify ECL signal.The ECL immunosensor was used to detect Brom with a low detection limit of 1.5 pg m L^-1.In chapter 3,the CdSe@SiO₂ synthesized by reverse microemulsion method is an ideal platform for multifunctional biological probes.The ECL immunosensor was used to detect SAL with a low detection limit of 0.17 pg mL^-1.In chapter 4,CdSe QDs,AuNPs,CdSe@SiO₂ and PAMAM-SNR were characterized by TEM,SEM,UV spectra,IR spectra,zeta potential and dynamic particle size distribution.