Fabrication And Cells Adhesion Of PDA Modified PNIPAM

As a well-known method in the polymer synthesis,Plasma polymerization used plasma sources to generate kinds of “activity” by gas discharge,providing energy to polymer monomer,and then forming polymeric film on a diversity substrate by the reaction between monomer and “activity”.Plasma polymerization is a single-step reaction,initiator free,pinhole free and highly dense to produce thickness polymeric films.As a kind of smart materials,PNIPAM is one of the most popular studied temperature-sensitive materials,with the lower critical solution temperature(LCST,37 oC)was closed to physiological temperature.By this LCST,allows PNIPAM has potential application prospects in drug delivery,cells culture and tissue engineering,etc.In this paper,temperature-responsive films of Poly(N-isopropyl acrylamide)(PNIPAM)were fabricated by plasma initiated polymerization in one-step.The composition and the interaction between the components were characterized and detected by FT-IR and X-ray photoelectron spectroscopy(XPS).The temperature-responsive properties of the films were investigated and expressed by water contact experiments.The morphology of the fabricated films and the roughness that within microtopography were observed and confirmed by SEM detection.Moreover,a crosslinker of N,N’-Methylene bisacrylamide(MBA)could effectively control the roughness of the fabricated PNIPAM films.Furthermore,the cell adhesiveness of the films with roughness to Hela cells were investigated.The results indicated the significant potential applications of such plasma initiated responsible films with rough surfaces and adhesion ability to cells in medical treatment and disease detection or diagnostics.PNIPAM was functioned and modified by co-polymerized of poly(dopamine)(PDA)coatings for surface modification in biomaterials or for other subsequently functional surface modification.Co-polymerization of PNI-PDA by assembly was fabricated,and then,the composition and the interaction were characterized and detected by FT-IR and XPS.PNI-PDA retained both the PNIPAM structure and the PDA functional group.The morphology of the PNI-PDA films and the roughness that within microtopography were observed and confirmed by SEM,PNI-PDA films had the same morphology and roughness with the PNIPAM films.The thermostability of PNIPAM and PNI-PDA were detected by DSC-TGA.Furthermore,the cell adhesiveness of the PNI-PDA films to Hela cells were investigated and compared with PNIPAM films.