Study On Cu(?)Ni(?) Sensor In Bioelectrochemical System

For the heavy metal waste water in the environment,through the bioelectrochemical system for treatment,degradation of heavy metal ions at the same time,but also produce hydrogen,methane and other clean energy,is a very promising approach,mainly the use of anode production of microorganisms,To the pollutants in the sewage as a nutrient,through its own metabolic activities,produce electrons and pass to the cathode,heavy metal ions to be electron reduction reaction,which can be removed.However,there are many ways to monitor heavy metal ions,which are well developed biosensors.The principle is to use a biologically sensitive element that,for a change in concentration,produces a corresponding electrical signal to detect the instrument,of the biometric sensor as a recognition element,but also need a kind of electrical signal amplification analysis of the physical and chemical converter,to complete the entire identification process.The research of this subject is to explore,bioelectrochemical system in the degradation of heavy metal ions at the same time,do not need any physical and chemical converters,through the production of electrical signals,dedicated to the application of biosensor monitoring.In this experiment,the concentration of heavy metal ions in the influent was changed by single-cell microbial fuel cell(SMFC)and single chamber microbial electrolytic cell(SMEC).The electrochemical parameters were observed by a series of electrochemical data trends,analytical biosensor as a standard curve,after the sensor will be optimized,as follows:(1)the first part of the first through two types of reactor start domestication,in SMFC,when the reactor started successfully,the voltage maintained at about 0.6 V,adding 1 mg/L copper solution to the system for domestication,in the process,To measure some of the electrochemical and physical and chemical data,to be stable after the reactor operation,change the concentration of copper ions in the water to observe the overall trend of changes in electrochemical data;for SMEC this part,because SMEC is completely anaerobic state,the reactor was sealed,and the SMEC requires additional power(0.3 V),the processing of heavy metal ions is relatively low redox potential of nickel ion,was added 2 mg/L solution containing nickel acclimation phase,in the process,measurement Some electrochemical and physical and chemical data,until the reactor stable operation,by changing the concentration of nickel ions in the water,to observe the overall trend of changes in electrochemical data.(2)The second part is the analysis of the above electrochemical data,the maximum R2 value of the analytical curve,as the standard curve of the sensor for qualitative and quantitative analysis.In the SMFC reactor,the electrochemical response is obtained by changing the concentration of Cu(?)solution,which is divided into voltage,current density,power density,anode potential and cathode potential,where the voltage and concentration are linear.In the SMEC reactor,by changing the concentration of Ni(?)solution,the electrochemical response,divided into voltage,current density,power density response,the general law and SMFC similar.(3)The last part is the optimization of the sensor monitoring conditions and the analysis of the structure of the anode microbial community,in which the SMFC sensor is optimized for the parameters,including COD,pH,buffer solution PBS,etc.The optimal monitoring conditions are as follows: COD = 800 ~ 1100 mg/L,p H = 6 ~ 6.5,R = 800 ~ 1000 ?,PBS = 100 ~ 120 mM.SMEC sensor is no exception,the COD,pH and other parameters of the optimization,the optimal monitoring conditions are as follows: influent COD = 800 ~ 1100 mg/L,pH = 5.5 ~ 6,external voltage = 0.4 ~ 0.5 V.In the case of SMFC / SMEC reactor,the microbial community structure of the reactor anode can be used to determine the classification of the species on the anode biofilm,and the dominant species in the classification.The predominantly Geobacter plays an important role in the generation of electrons and the removal of contaminants.