Experimental Study On Toxicological Safet Evaluation Of Pyralis Farinalis–Litsea Coreana Insect Tea

Object:In order to examine the safety of Pyralis farinalis –Litsea coreana Insect Tea in toxicology,a series of tests are performed,including the acute and subchronic toxicity test,genotoxity and teratogenicity,according to Procedures and methods for toxicological assessment of food safty(GB 15193.1-2014)Methods:1.Acute oral toxiciy test : Healty adult KM mice(10 male and 10 female)were selected for this test.Mice were treated once by Pyralis farinalis –Litsea coreana Insect Tea 30 g/kg·bw through stomach and consecutively observed for 14 days.Record toxic effect and death of mice during the course of the test.2.Bone marrow micronucleus test: 50 healthy KM mice(half male and half female)were orally administered with Pyralis farinalis –Litsea coreana Insect Tea extract by 24 h intervals method at the dose of 10 g/kg·bw?5 g/kg·bw?2.5 g/kg·bw respectively.Cyclophosphamide(CP)was used via intraperitoneal injection at a dose of 40 mg/kg ? bw as concurrent positive control and sampled on 6 h after the second dosing.The slides were scored for micronuclei and for polychromatic(PCE)to normochromatic(NCE)and the ratio of polychromatic erythrocytes to normochromatic erythrocyte.3.Dominant lethal assay test: Pyralis farinalis–Litsea coreana Insect Tea extract was administered to male mice with doses of 10 g/kg·bw?5 g/kg·bw?2.5 g/kg·bw separately for 5days in succession.Cyclophosphamide(CP)was used via intraperitoneal injection at a dose of 40mg/kg ? bw as concurrent positive control,then,these male mice mated with female mice(1:2)for five weeks consecutively.Females were sacrificed on the17th-20 th day from the first administered day,the mumber of embryo implantated,live births and stillbirths were recorded.All data were evaluated by appropriate statistical methods.4.90-day feeding test: 80 healthy SD rats(half male and half female)were divided into four groups and were gavaged with Pyralis farinalis –Litsea coreana Insect Tea at different doses of 15g/kg·bw?10 g/kg·bw?5 g/kg·bw,respectively once a day for 90 days.At the end of the test,all the animals were sacrificed.The body weight and organ weight of all animals were recorded,and their organ coefficients were counted.The rountine blood and biochemical indexs of all animals were measured by automated hematology analyzer and biochemical analyzer respectively.The changes of gross anatomy and histopathology were observed by visual and microscope observation in rats.5.Teratogenic test: 100 healthy SD female rats were free to mate with 50 SD male rats as the ratio of 2:1 respectively.Pregnant rats were orally treated once a day with Pyralis farinalis –Litsea coreana Insect Tea extract at three does of 15 g/kg·bw?10 g/kg·bw?5 g/kg·bw,respectivly during pregnant 6 th ~ 15 th days.All female animals were sacrificed at the 20 th day.The embryo toxicity index of pregnant rats and the growth index of fetal rats were anatomically observated respectively.Results1.There weren't toxic symptoms and death to take place for all groups of animals in acute oral toxicity test.LD50(male and female mice)>30 g/kg ? bw.This sample belonged to no toxic class based on class method of acute toxicity median legal dose.2.Bone marrow micronucleus test: Pyralis farinalis –Litsea coreana Insect Tea is not mutagenic in all dose levels.40 mg/kg ? bw CP have a higher micronucleus rate relative to negative control.Pyralis farinalis –Litsea coreana Insect Tea is not mutagenic in mice bone marrow micronucleus assay.3.The results indicated that there were no significant differences among treated groups and the negative-control group(P> 0.05),no siginaificant mutagenic activity in dominant lethal text,at 10g/kg ? bw,Pyralis farinalis –Litsea coreana Insect Tea didn't produce significant effects on the reproductive parameters of litters and fetal growth,and did not induce the teratogenic effects in fetuses.The genetic toxicology above indicated no evidience that Pyralis farinalis–Litsea coreana Insect Tea was mutagenic and terarogenic potenrial for animals.4.The results of 90 days feeding study indicated Pyralis farinalis –Litsea coreana Insect Tea had no harmful effect on these indexes of blood,clinical chemistry,organ weight,organ coefficient.Histopathology observation proved that liver,spleen,kidney,stomach,spermary and ovary of tested animals had no significant changes.However,compared with the rats in control group,body weights of male and female rats in 15 g/kg ? bw group exhibited a significant increase.5.Teratogenic test : The results of teratogenic test indicated Pyralis farinalis –Litsea coreana Insect Tea had no harmful effect on these indexes of the body growth,the numbers of embryo implantation,body weight and lenght,tail length,the incidence of stillbirth and absorptive embryo,no obvious teratogenicity or reproductive toxicity.Conclusions:Pyralis farinalis –Litsea coreana Insect Tea did not show a positive response in mice,the acute and subchronic toxicity test indicating LD50(male and female mice)>30 g/kg ? bw.This sample belonged to no toxic class;dominant lethal test and bone marrow micronucleus test indicating that the possibility of genetic toxicity was small.90-day feeding test showed that Pyralis farinalis–Litsea coreana Insect Tea maybe aroused body weight loss compered with the congroup,no harmful effect on these indexes of blood,clinical chemistry,organ coefficient.Teratogenic test indicated the incidence of stillbirth and absorptive embryo,no obvious teratogenicity or reproductive toxicity.