Synthesis Of Small Molecular Sulfide Organic Fluorescent Probes And Its Properties

Fluorescent probe has been used in the fields of environmental science,biochemistry,medicine,materials science and so on because of its simple operation,high sensitivity,good economy.It is divided into fluorescent groups,identification groups and connecting part.The fluorescent groups and recognition groups played major roles.The combination between recognition group and analyte results the change of the fluorescence group's signal,which makes a change of the spectral properties.So,the purpose of detecting the target molecule is achieved.The using of fluorescent probes to detect sulfur-containing compounds has received increasing attention and research.In this paper,three fluorescent probes were designed and synthesized.We designed and synthesizes the probe molecule YA by choosing imidazo [1,2-a]pyridine as the fluorescent group and bromoacetyl as the recognition group.Using 1-methyl-1H-indole as the fluorescent group and the aldehyde group as the recognition group designed and synthesized the probe molecule YD.Using the imidazo [1,2-a]pyrimidine as the fluorescent group and the aldehyde group as the recognition group designed and synthesized the probe molecule YC.The chemical structures were characterized by 1H NMR,MS,IR and 13 C NMR.We studied the mechanism and characteristics of the probe compounds for thiourea and thiol by UV absorption spectra and fluorescence emission spectra.We investigated the effect of the probe spectrum in the cases of different solvents,different analyte concentration,different interferences.The Gaussian software was used to calculate the molecular configuration of the probe before and after identification,we get the corresponding molecular orbital information.The results showed that the probes could respond to sulfide with changes of the fluorescence signal.Probe YC could recognize L-cysteine selectively with the concentration of 1 ?M and MeOH/H2 O as a medium.Simulation results found that,there were differences in the distribution of electrons inside the molecule because of its structure,which resulted in a difference of fluorescence spectral shift andfluorescence intensity.The calculation of density functional theory explained the spectral behavior between the probe and the sulfide reasonably.