Self-Assembly Of Hydrophobins On Polypropylene Surface And Its Application In Drug Carrier

Hydrophobins are small secreted proteins that fulfil a broad spectrum of functions in fungal growth and development.They are small molecular weight proteins that exhibit both hydrophilic and hydrophobic groups.The most characteristic feature of hydrophobins is that their ability to self-assemble at the hydrophilic/hydrophobic interface to form an amphoteric membrane,changing the wettability of the surface of the material.Fungal hydrophobins are important for the growth and development of fungi,and they also have many potential applications.In this paper,a series of studies have been conducted on the application of rHGFI in surface modification and drug carrier.The protein membrane of rHGFI was prepared by self-assembly on the surface of polypropylene.The contact angle test results show that the presence of rHGFI film greatly improves the wettability of polypropylene surface,the non-characteristic adsorption of the surface of the polypropylene after rHGFI modification is also reduced.After XPS analysis,it was found that this adsorption is not attributable to chemical covalent bond,only a simple physical adsorption.This adsorption makes some changes in the roughness of the polypropylene surface,and in the SEM it is evident that the smooth surface of the polypropylene becomes uneven after being modified by rHGFI.This change makes the biocompatibility of the polypropylene surface improved,promoting cell adhesion,allowing cells to spread more easily on their surface.The rHGFI-Cur and SPI-Cur drug delivery system was prepared by vacuum freeze-drying and the fungal hydrophobin rHGFI and soybean protein isolate(SPI)were used as the carrier of curcumin(Cur).In the stability analysis,it was found that rHGFI and SPI had a promoting effect on the dispersion of Cur in aqueous solution,and the homogeneity and stability of Cur were better in rHGFI aqueous solution.rHGFI-Cur and SPI-Cur drug loading system reduced the Cur water diameter from 2.49 μm to 193.14 nm and 269.72 nm,the Zata potential value changed from-14.6 mV to-20.3 mV and-18.6 mV,this also proves that the stability of SPI-Cur and rHGFI-Cur is better than Cur,which is easier to be uniformly dispersed in aqueous solution.TEM test results can show up in the rHGFI-Cur drug delivery system in the least agglomeration,particle size distribution is more concentrated.The drug loading rates of rHGFI-Cur and SPI-Cur drug delivery systems were 4.11%and 3.08%,and the encapsulation efficiencies were 13.36%and 9.19%.Both drug loading systems showed significant sustained release properties in the physiological fluids,and the release of the rHGFI-Cur system was more complete than that of the SPI-Cur and rHGFI-Cur systems.The survival rate of TE-1 after rHGFI-Cur and SPI-Cur treatment was lower than that of Cur treatment alone,and the survival rate of rHGFI-Cur treated cells were the lowest when the drug concentration and duration of action were the same.As a new drug carrier,rHGFI drug delivery system is simple,non-toxic and biodegradable,and as an anti-tumor drug carrier can play a synergistic effect with drugs,and greatly improve the anti-tumor activity of drugs.