Effect Of Active Small Molecules In The Extracellular Of Strain NY3 On Alkanes Degradation And Its Mechanism

Pseudomonas aeruginosa NY3 is an excellent hydrocarbon-degrading strain,Pseudomonas aeruginosa NY3 is an excellent hydrocarbon-degrading strain,in addition to a variety of start-up hydrocarbon degradation oxidase,which has a very rich extracellular secretions,the effect of other extracellular secretions other than Rha on the degradation of hydrocarbons by this bacterium is rarely reported.In this paper,the effect of coexisting organic acids on the degradation efficiency of NY3 bacteria was studied.The main reason for the effect of coexisting organic acids on the degradation efficiency of Pseudomonas aeruginosa NY3 was studied by affecting the extracellular secretions of the bacteria,especially phenazine The amount of material secretion.In this paper,NY3 strain was used as the tested strain,and the single alkane was used as the degradation object.The following results were obtained by means of UV spectroscopy,gas phase,flight mass spectrometry and electron paramagnetic resonance.(1)Coexisting glutaric acid,succinic acid,malonic acid,acetic acid and oxalic acid can promote the degradation efficiency of tetradecane to NY3 bacteria compared with hexadecane as the sole carbon source,in which glutaric acid and O xalic acid is promoted for more pronounced.In addition to oxalic acid,other coexisting organic acids can promote the growth of NY3 bacteria.(2)The growth of Escherichia coli in Escherichia coli was higher,and the degradation efficiency was at least 72 h.The degradation efficiency of extracellular fluid to hydrocarbons is closely related to the two oxidoreductases(NADH and GSH)and phenoxine-based redox activators secreted by NY3 strain.(PHZ),pyocyanin(PYO),phenazine 1-formamide(PCN),phenazine 1-carboxylic acid(phenolic acid)(PCA),the growth of carbon sources can affect the secretion of phenazine compounds.(3)Coexisting oxalic acid was detrimental to the reproductive ability and cell degradation of NY3 cells.However,coexisting oxalic acid could significantly promote the degradation efficiency of nisalane in NY3 strain.Studies have shown that coexisting oxalic acid increases the efficiency of hydrocarbon degradation by promoting the amount of PC3 secreted by NY3.PCA secretion was positively correlated with the degradation rate of extracellular fluid to tetradecane.Cell degradation of hydrocarbons at the same time,the extracellular liquid on the degradation efficiency of hydrocarbons can not be ignored.Coexisting oxalic acid can increase the degradation rate of tetradecane by about 28%.O xalate co-metabolism can promote the degradation of PCA in the extracellular fluid of NY3 cells to accelerate the degradation of hexadecane by the free radical mechanism of extracel ular fluid.(4)The results of in vitro degradation showed that PCA,PYO,PCN and PHZ secreted by NY3 could react with the extracellular oxidative reduction coenzyme NADH and GSH to degrade tetradecane,Tetraalkane removal rate up to 48.1% in 8 hours..Phenazine and coenzyme NADH and GSH can produce hydroxyl radicals,and extracellular fluid and its phenazine degradation of hydrocarbons is caused by these hydroxyl radicals.The results of this study lay the theoretical foundation for the optimization of NY3 bacteria to repair environmental conditions of petroleum hydrocarbon pollution and its application.