Study On Biological Functions Screening Of Rapeseed Active Peptides And Its Application Research

Rape is the largest oil crop in China and its oil production accounted for more than 40% of total oil production.As byproduct of rape,rapeseed meal product more than 14 million tons annually.The proteins content of rapseed meal up to 30-42%.Low-cost rapeseed meal can be used for the preparation of high value-added active peptides.Our research group has already made the rapeseed active peptides industrialized successfully.The rapeseed active peptides products are welcomed by consumers.They respond several biological functions which were not concerned during product-development period.Therefore,the basic research work of the related biological functions screening need to be carried out urgently.Based on this requirement,the biological function of rapeseed active peptides will screened in 6 demends.They are improving slumber,lowering cholesterol,hypoglycemic and hypolipidemic,antihypertension,antioxidant and accelerate BRL calls proliferation.This work will systematically exploit and screen biological functions of rapeseed active peptides.Consequently,this research would provide basic and technical support for the development of efficient high quality rapeseed active peptides terminal products.(1)Biological functions screening was conducted to rapeseed active peptides.In improving slumber test,rapeseed active peptides significantly increased the sleep rate of mice by 30% and prolonged the sleep time of mice by 972 s.In the cholesterol-lowering test,the rapeseed active peptides could not significantly bind the cholic acid salt.In the hypoglycemic and hypolipidemic test,rapeseed active peptides could not significantly reduce the fasting blood glucose,total cholesterol and triglyceride content in mice serum.In the antihypotension test,the rapeseed active peptides had the effect of significantly reducing SBP and DBP.The SBP maximum reduced 17.8 mm Hg at 6 h after intragastric administration,and the DBP maximum reduced 12.3 mmHg at 6 h after intragastric administration.In the antioxidant test,the rapeseed active peptides did not significantly increase the content of SOD,GSH-Px or reduce the content of MDA in mice.In the BRL cell proliferation test,rapeseed active peptides could significantly promote the proliferation of BRL cells at the concentration of 0.5 mg/mL for cultivating 12 h.The proliferation rate was up to 56.98%.The result indicated that rapeseed active peptides were effective in biological functions of improving slumber,antihypertension,accelerate BRL calls proliferation.Besides,rapeseed active peptides have no effect biological functions of combining bile salts,hypoglycemic,hypolipidemic or anti-oxidation.(2)Chinese materia medicine gastrodia and schisandra played an important role in improving slumber.But they had side effects if used with high-dose.The results of experiments forward showed that rapeseed active peptides had the effect of improving sleep rate and prolonging sleep time on mice.In this study,the rapeseed active peptides were added into gastrodia and schisandra with the equal quality dose to study synergies effect of rapeseed active peptides and Chinese materia medicine.The results of experiments illustrated that rapeseed active peptides-gastrodia compound preparation and rapeseed active peptides-schisandra compound preparation could significantly shortening mice sleep latency of 42.7% and 32.6%,while prolonging mice sleep time of 77.9% and 87.2%.Adding rapeseed active peptides into gastrodia and schisandra could maintain improving sleep effect while reducing the quantity of Chinese materia medicine.(3)As an antihypertensive drugs,captopril declined blood pressure rapidly and range-largly.However,the antihypertension effect could not maintain for a long time.The experiments forward showed that rapeseed active peptides has a good effect on maintaining antihypertension effect for a long period.In this study,the rapeseed active peptides were added into captopril with the 50:1 quality ratio dose to study synergies effect of rapeseed active peptides and captopril.The results illustrated that all of rapeseed active peptides,captopril and theirs compound preparation had valid hypertensive effect.Using rapeseed active peptides and captopril together was better than using individually on antihypertensive effect.The SBP reached maximum reduction of 24.5 mmHg at 6 h after intragastric administration in single-gavaged test,while the SBP in long-term gavaged test reached maximum reduction of 27.3 mmHg at the 3 week.The target acting organs are arteries,lungs and kidneys.Serum indexes showed that rapeseed active peptides-captopril compound preparation conduct antihypertensive effect maily through descending content of ET-1,Renin,ANG II and ascending content of NO,eNOS,which were antihypertensive-related serum indexes.It has better activity on ascending and descending those antihypertensive-related serum indexes.Adding rapeseed active peptides into captopril could both maintain blood pressure decline amplitude and prolong effective time.Meanwhile,the using quantity of captopril could reduce.