Preparation Of Spiropyran Light Response Drug Carrier And The Study Of Its Properties

Chapter 1: The types of materials used to construct drug carriers and the stimuli responsive drug carriers and their research progress were reviewed.Meanwhile,the structure,properties,applications and research progress in light responsive drug carriers of spiropyran were summarized.Chapter 2: SPOH and SPCOOH were synthesized by using2,3,3-trimetyl-3H-indolium bromide and 5-nitrosalicylaldehyde as the main materials.The structures of them were characterized by 1H NMR and FTIR,and the optical properties of them were compared by UV-Vis spectroscopy.The spectral data showed that in the same concentration of ethanol solution,UV light irradiation 1000 s later,the UV absorption intensity of SPOH in the550 nm wave is about 0.5,while the SPCOOH is about 0.9.At the same time,When the material is restored to its original structure under visible light irradiation,the time required for SPOH is 1000 s,while SPCOOH is 500 s.The results show that the spiropyran with carboxyl group(SPCOOH)has better photochromic properties,which provides a better choice for the application of spiropyran in photoresponsive drug carriers.Chapter 3:SPCOOH-β-cyclodextrin were synthesized by esterification with SPCOOH and β-cyclodextrin as the main materials.The structure of SPCOOH-β-cyclodextrin was characterized by FTIR.DOX as a model drug,SPCOOH-β-cyclodextrin was used as light responsive drug carrier,and the optical response of the carrier was studied by fluorescence spectroscopy.It was found that the fluorescence intensity of adriamycin in the solution increased from 80 to about 110 after 300 s of visible light irradiation.When the solution was irradiated under UV light for 180 s,the fluorescence intensity of DOX decreased from 110 to 75.The results show that the drug carrier is a novel light-responsive drug carrier,that visible light can promote drug release.Chapter 4: Mesoporous silica nanoparticles(MSNs)were synthesized by sol-gel method with TEOS and CTAB as the main materials.The morphology of this particles was observed by transmission electronmicroscopy(TEM),nitrogen adsorption/desorption and X-ray diffraction.The results show that the structure of this mesoporous silica particles is a typical MCM-41 structure and its specific surface area,the pore volume and the pore volume is 1001m2/g,1.31cm3/g,2.7nm,respectively.Subsequently,the surface of the mesoporous silica particles was aminated with a 3-aminopropyltriethoxysilane coupling agent,and then SPCOOH-MSNs light-responsive drug carrier is obtained by dehydration condensation with aminated mesoporous silica and SPCOOH as the main materials.Under three conditions(ultraviolet light,visible light and darkness),the cumulative release value of doxorubicin DOX(model drug)in this system was studied by UV-Vis spectroscopy.Under UV irradiation,the drug release rate of this drug carrier was about 74% within 20 min,while in the visible and dark conditions,the drug release rate was only about 23% and16%,respectively.