Separation,Purification And Analytical Identification Of The Saponins From Ornithogalum Caudatum Ait

The separation,purification of Ornithogalum Saponins and analytical identification were studied in this paper.Ornithogalum saponins were extracted though water extraction,and the extracted conditions were optimized:the optimum boiling time was 3h;water quantity was 15 times of the raw material;extracted for 2 times.Ornithogalum saponins in different parts were extracted,and the result was showed:the extraction rate of the root was 0.94%,the stem was 0.89%,the leaves was 0.85%.The total saponins of the different parts were detected by HPLC,the results showed that the most saponins were contained in the stem.The yield of Ornithogalum saponins was 1.35%By n-butanol extraction.AB-8 and D296 macroporous resin used to remove sugar and color of the crude products.The yields of products were respectively 0.51%and 0.14%relatived to the raw materials.Ornithogalum saponins were separated and purificated by using silica gel column chromatography and solvent chloroform and methanol.The yields of monocomponent were 1.83%,2.67%,3.17%,5.33%,15.33%,5.67%,4.21%.By HPLC each component was detected,the results were 60.69%、65.01%、69.89%、73.31%、81.76%、77.22%、70.73%。Screening to the conditions of caudaside-glycosidase,used 25%lixivium of Caudaside as revulsant,Absidia sp.048 and Absidia sp.0848 were selected to react to Ornithogalum saponins,and the component E of the saponins were transformed.