Extraction Of Pig's Tendon Collagen And Study On Protein-based On Packaging Materials

Pig's tendon is the main byproduct of pork,which is rich in resources.The tendons are rich in protein and are considered as good raw materials of collagen and gelatin.In order to make use of the resource of animal tendons,improve the added value,the collagen is extracted from pig's tendons with enzymatic extraction.The main research contents are as follows:Firstly,this research was based on removing fat and the facts of extraction process: enzyme amount(?/g),time(h),temperature(?),pH,ratio of material to liquid.The extraction rate of the collagen was studied by single factor process.Through the orthogonal experiment,we could get the optimum condition for extraction.The experimental condition was that enzyme amount was 250?/g,time was 25 h,temperature was 25?,pH1.6,ratio of material to liquid was 1:20.The yield was 45.8g collagen per 100 g of defatted pig tendons.In this paper,the factors of the process were investigated,and the optimum experimental condition was finally determined.Factors affecting the extraction of sorting: enzyme>temperature>ratio of material to liquid>p H>time.The collagen was identified to be type I collagen and the denaturation temperature was 49 °C.Besides the extraction process of the pigs' tendon,there were these advantages: saving time,saving energy and so on.The process conditions of forming pigs' tendon collagen edible film were discussed too.We studied the influences of protein concentration,glycerol concentration,heating temperature on film formation.The single factor experimental results showed that the membrane mechanical strength and the tensile property increased with increasing in collagen concentration in the film forming solution,but when the collagen concentration was too high,it could affect the solubility of film,the optimum collagen concentration was 6g/100mL;When the heating temperature was 40?,denaturation of proteins occurs most favorable to film network structure;The add of the plasticizer glycerol's was helpful to enhance the film flexible,but when its concentration was too high,it would be unavailable to taken off,the optimum glycerol concentration was 2g/100 m L;When PH was 3,the film mechanical strength and the tensile property were fine.The orthogonal experimental results showed that the superior technological conditions are: The collagen concentration was 6g/100 mL,the heating temperature was 40?,the glycerol concentration was 2g/100 m L.Finally,the preparation process of the pig's tendon collagen sponge and its related performance were as follows: Through the single factor experiment,the concentration of collagen,glycerol,and thickness was considered to prepare collagen sponge.Then adsorption performance for ethanol was studied by the factors of porosity and time.When the concentration of collagen,glycerol was 1%,0.6% and thickness was 5mm,the tensile strength was 210.91 KPa and the elongation at break was 6.98%,the porosity of sponge was 91.19%,the adsorption rate for ethanol was 416.32mg/g after 30 days.Besides the adsorption rate was positively correlated with the porosity of the sponge.After some cushioning performance tests found that: the sponge was only applicable to pack lighter goods.Besides,repeated compression and recovery performance was extremely good.