Synthesis Of PNA And Construction Of New Ligands For Hybrid Catalyst

Peptide nucleic acid(PNA)is a nucleic acid mimics which have very dramatic and excellent advantages and it has been applied to broader applications.It has showed preferable abilities in construction of molecular biology tools and many other areas.Now some researchers even think it has played some role in the origin of life.Chiral catalyst based on the biological macromolecules has been a hot research topic in recent years.DNA based hybrid catalyst is the most promising catalyst which have many related reports.The effective results may be explained by the chiral environment supported by DNA,but the detailed mechanism has not been figured out yet.The new ligand for the DNA hybrid catalyst is the key for the explaination of the catalytic mechainism and may give an opportunity to construct a new catalyst.Although PNA has many advantages which may just satisfy the needs of the new ligand,it has not been applied in this field.Start from the synthesis of PNA,we have synthesized PNA backbone and four kinds of carboxylic acid derivatives.Four kinds of PNA monomers based on the Boc/Z protect group strategy were prepared through the amide condensations.The preparation of T monomer need 3 steps and it take 4 steps to prepare A and C monomers.The synthesis of G monomer is the most complex and it take 6 steps while we optimized the path slightly.We tried the solid-phase synthesis and the liquid synthesis of PNA short chain.Finally,we choose the liquid synthesis to synthesized the T dimer and T trimer short chains.We also synthesized the carboxylic acid derivative of bipyridine and connect it to the PNA T monomer and the short chains to build the elementary new ligands for the hybrid catalyst.We finished the most works to construct a new hybrid catalyst,which will have very important significance fot the explaination of mechainism of hybrid catalyst and may contribute to the design of new catalysts.