Transmembrane Transport Of Multicomponent Liposome-Nanoparticles Into Giant Vesicles

Multicomponent Liposome-Nanoparticles are a novel gene carrier that is composed of nanoparticles with a concentration of multicomponent liposomes.And nanoparticles dispersed in solution with self-assembled nanoparticles and lipid shells.The internal core is composed of the polymer,the equivalent of a nanoparticles;the outer shell consists of lipid layer.The multicomponent liposome-nanoparticles vector can accurately deliver and then discharge the gene of interest into the target cell,which is very popular in the field of biomedical gene therapy.Giant vesicles are lipid bilayers that are rolled into spherical shells and are widely used to study the properties of lipid bilayers.In this paper,we studied the transport of multicomponent liposome-nanoparticles across the membranes of giant vesicles in solvents using the self-consistent field theory.In the first chapter,we introduced the structure and characteristics of cell membrane,the synthetic membrane,the structure,preparation and application of multicomponent liposome-nanoparticles,and the mode of action of nanoparticles and cell membrane.At the same time,the application of self-consistent field theory model in the cell membrane system is introduced.In this paper,we studied the self-organization behavior of cell membrane through self-consistent field method.Inthesecondchapter,westudiedthetransportofmulticomponent liposome-nanoparticles across the membranes of giant vesicles in solvents using the self-consistentfieldtheory.Basedontheanalysisofthemulticomponent liposome-nanoparticles permeating the giant vesicles membranes,a simple transport model is proposed.We discuss the effect that the difference in membrane morphology induced by variation in the head volume fractions of multicomponent liposomes and the size of the nanoparticle has on endocytosis.We also examine the role of energy barriers in quasiequilibrium.When the head volume fractions of lipid species A(f_{h A})and B(f_{h B})were the same,we obtained metastable intermediates of the IMI,H_II,stalk and HD phases,whereas when the head volume fractions of lipid species A and B were different,metastable intermediates of the stalk,IMI,SUV,H_II and HD phases were formed.However,the radius of the nanoparticle（R_p）has little effect on the membrane morphology.We calculated the minimum free energy paths using the free energy curve.The optimal parameter combinations obtained by comparing free energies are f_{h A}=f_{h B}=0.2,f_{h A}=0.2,f_{h B}=0.55,and R_p=0.35R_g.These results indicate that interaction between multicomponent liposome-nanoparticles and giant vesicles is a spontaneous process and the energy barrier has to be overcome to form metastable intermediates.In the third chapter,we studied the transport of multiple multicomponent liposome-nanoparticles across the membranes of giant vesicles in solvents using the self-consistent field theory.Based on the analysis of the multiple multicomponent liposome-nanoparticles permeating the giant vesicles membranes,a simple transport model is proposed.We discuss the effect that the difference in membrane morphology induced by variation in the number of the multicomponent liposome-nanoparticles and the arrangement of the multicomponent liposome-nanoparticles has on endocytosis.We also examine the role of energy barriers in quasiequilibrium.We calculated the minimum free energy paths using the free energy curve.These results indicate that interaction between multiple multicomponent liposome-nanoparticles and giant vesicles is a spontaneous process and the energy barrier has to be overcome to form metastable intermediates.In the fourth chapter,we summarize the results of the transport of multicomponent liposome-nanoparticles across the membranes of giant vesicles in solvents.