Compound Stomach Stagnation Capsule Preparation Research

Compound stomach stagnation capsule consists of six kinds of Chinese medicinal atractylodes,immature bitter orange,hawthorn,Costustoot,nutmeg,Picrorhizae.This topic for Compound stomach stagnation capsuledrug preparation process,quality standards,pharmacological effects determine have carried on the systematic study,A preparation process was optimized,determine the quality standards,and its pharmacological efficacy is gived.First of all,Compound stomach stagnation capsule preparation process choose material liquid ratio,extraction time,infiltrating time three factors of Costustoot and nutmeg volatile oil extract in nutmeg steam distillation process optimization;Choose materials liquid ratio,extraction time,temperature three factors on the hawthorn organic acids extract on the single factor investigation,orthogonal process optimization;Choose materials liquid ratio,extraction time,temperature and concentration of ethanol four factors of atractylodes,immature bitter orange,Picrorhizae on the single factor investigation,orthogonal process optimization.secondly,by investigating the moisture,ash content,extract content and organic acids,atractylodes lactone I,naringin,hesperidin,new hesperidin,Picrorhizae glycosides I content determine its quality standard.Finally,pharmacological experiments adopt irregular feeding,through the ink surface FenHu to test the effect of gastrointestinal propulsion.Experiment results show that,the best extraction technology for Costustoot,nutmeg:Material liquid ratio of 1:10(g/mL),extracting time 5h(keep micro boiling),Under this condition of the volatile oil content is 1.80 mL.The best technology of hawthorn water extraction is: the material liquid ratio of 1:20(g/mL),extraction time2.5h,temperature 80 ?,under this condition of the organic acid content is7.4062%.The best technology of Atractylodes,immature bitter orange,Picrorhizae alcohol extraction of the volatile oil content is:the material liquid ratio of1:15(g/mL),extraction time2.5h,temperature100?,80% ethanol concentration,on this condition the youpigan of 1431.13ug/g,675.37ug/gbaizhuneizhiI,huhuanglianganI is3614.21ug/g.Quality standard to determine the experimental results showed,Compound stomach stagnation capsuleof the total ash content was 4.18%,with 50%ethanol soak extract content is 91.77%,organic acid content is 7.2%,baizhuneizhiI to0.126 mg/g,youpigan is 1.006 mg/g,chengpigan was 4.436 mg/g,xinchengpigan was2.196 mg/g,huhuanglianganI is 0.273 mg/g.Volatile oil contains 83 kinds of ingredients.Pharmacological experiments,compared with control group,high dose group of small intestine propulsion rate and the residual rate of gastric contents had significant difference(P < 0.05),medium dose group of small intestine propulsion rate have significant difference(P < 0.05),Compared with positive control group,high dose group of small intestine propulsion rate and the residual rate of gastric contents had significant difference(P < 0.05).Preparation process optimization results were higher than the single factor experimental results,in line with the verification experiment,determined to be the best process,determine the feasible quality standards at the same time.Preliminary pharmacological experiments confirmed that Compound stomach stagnation capsule can increase the rate of FD intestinal propulsion in mice,decrease the rate of gastric contents residue,can improve the symptoms of FD,the efficacy of the spleen.