| Hexafluorobisphenol A, bisphenol AF ?BPAF?, is a class of emerging bisphenols. Due to the limitation of bisphenol A ?BPA?, BPAF is widely used as a substitute for BPA, and BPAF is detected in various environmental media. The study of BPAF neurotoxicity and neuroendocrine disrupting effects is important for assessing the environmental effects of these compounds: it is important to understand the toxicological effects of BPAF on aquatic organisms, which will be of great practical significance for BPAF ecological monitoring and ecological evaluation. The effect of BPAF on the fish has a neurological effect and its potential mechanism, which can provide a theoretical basis for the production and management of BPAF and provide a scientific basis for the ecological risk and health risk assessment of mammals ?such as humans?.In this study, we used the BPAF exposure experiment to study the macrophages from the macroscopic order to the individual level - the level of hormone - the molecular level.The study was carried out by using the T-maze to study the effect of hexafluorobis Phenol-A exposure to zebrafish learning ability,and then detected the number of adrenaline and cortisol hormone levels, the number of related gene expression and changes in the expression of the nervous system-related genes. In this study, we have obtained the following preliminary results:1. Zebrafish T-maze behavior study shows that BPAF has a potential learning and memory ability of zebrafish, which can cause the learning and memory ability of zebrafish adult fish to weaken. Mainly as follows: BPAF exposure can inhibit the normal operation of the adult zebrafish nervous system, so that the right time to enter the T-maze latency increased,and there was a significant difference: a significant dose-effect relationship,BPAF exposure dose higher, the higher the degree of learning and memory ability of fish,the effect on female zebrafish and male zebrafish was not significantly different.2.The levels of cortisol and adrenaline in the whole fish homogenate of zebrafish were detected by enzyme - linked immunosorbent assay ?ELISA? from the aspects of hypothalamic - pituitary - adrenal axis ?HPA? regulation. The effects of BPAF on the expression of HPA-related genes in zebrafish were detected by real-time fluorescence quantitative PCR,including corticotropin releasing hormone ?CRH? gene and adrenocorticotropic hormone ?ACTH? gene. ?1? high dose of BPAF exposure can cause elevated content of COR in female zebrafish homogenate. When the concentration of BPAF was 0.5 mg·L-1, the plasma COR content in the plasma was significantly higher than that in the control group. Similarly, exposure to BPAF can cause elevated levels of COR in male zebrafish homogenates. Among them, 0.05 mg·L-1 BPAF showed significant difference ?P<0.05?, 0.5 mg·L-1 ?P<0.01?. ?2? BPAF exposure can cause a decrease in EPI content in female and male zebrafish. Among the 0.5 mg·L-1 BPAF exposure group, the EPI content in female and male zebrafish was significantly lower than that in the control group ?P<0.05?. Compared with the change trend of COR, BPAF Exposure to inhibit the synthesis of EPI in zebrafish. ?3? The relative expression of crh in female and male zebrafish increased with the increase of exposure concentration. At the concentration of 0.5 mg·L-1, the expression of crh mRNA was significantly increased ?P<0.01 ?. ?4? The expression of acth mRNA in the brain of female and male zebrafish was inhibited by BPAF exposure. At the concentration of 0.5 mg·L-1, the expression of acth mRNA in female zebrafish was significantly decreased ?P<0.01?, male zebrafish acth ?P<0.05? .There was a significant decrease in the expression of acth mRNA in the brain with the increase of BPAF exposure concentration, but there were significant differences in the exposure group,and at the other exposure group,the expression of acth mRNA was not significantly different from that of the control group.3.The effect of BPAF on the expression of Nerve system-related genes in zebrafish was detected by real-time fluorescence quantitative PCR in the level of gene expression related to the nervous system of zebrafish. The results showed that: ?1? The relative expression of gfap gene was significantly up-regulated with the increase of BPAF exposure concentration. The expression of gfap in male zebrafish was significantly higher than that in control group ?P<0.05?, but the expression of gfap did not change significantly when the concentration of gfap was higher than that of control group ?P<0.05?, but when the concentration increased to 0.05 mg·L-1, gfap Of the relative expression of a significant reduction. ?2? In the expression of shha in female zebrafish, the expression of shha showed a decreasing trend with the increase of exposure concentration, and showed a significant difference when the concentration increased to 0.5 mg·L-1. In the expression of male zebrafish, the tendency to rise first and then decrease. ?3? The relative expression of syn2a in female zebrafish and male zebrafish was consistent, and the relative expression of syn2a decreased with the increase of BPAF exposure concentration. At 0.05 mg·L-1, the relative expression of syn2a was significantly decreased ?P<0.05?. The relative expression of syn2a was decreased at 0.5 mg·L-1, the significance level was P<0.01. ?4? The relative expression of pcdhl8b in female zebrafish and male zebrafish brain decreased with the increase of exposure concentration. The inhibitory effect of BPAF on pcdhl8b in females and males was significantly different at 0.5 mg·L-1 concentration. The results showed that BPAF exposure had a certain interference effect on the genes related to the nervous system of zebrafish. |
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