The Characteristic Study About The Bionic Fluorescent Material Adsorbing Bsa Spectrum/Interfacet

With the development of nano technology,nano fluorescent material has become one of the hot research,especially fluorescent silica nanoparticles because of its toxicity and is suitable for living and the most widely studied.However,the low loading rate of fluorescent silica nanoparticles limited the application of immobilization.Therefore,This topic with Dopamine as modifier,Sof as doping dye,TEOS as the silicon source,synthesized biomimetic fluorescent silica nanoparticles.And adsorb BSA as the carrier,researching about its own solid loading ratio,luminous performance,interface features.It provides a good platform for the application of fluorescent nanomaterials in the field of immobilization.Using reverse microemulsion method to made fluorescein sodium/silicon dioxide composite nanoparticles.Told its own structure and luminescence properties have been characterized by SEM,EDS,FT-IR,XRD,UV-Vis and fluorescence spectral characterization methods.SEM graph shows that the composite material is made by uniform spherical,Particle size of around 70 nm and good dispersion.XRD spectrum showed that the fluorescent silica nanoparticles is typical of the amorphous state;C,Na elements appear to prove the fluorescein sodium has been successfully be wrapped in EDS graph.In FT-IR spectrum,benzene ring skeleton stretching vibration peak,C=O stretching vibration peak appear show that sodium fluorescein was successfully wrapped in silica.UV-Vis spectra indicate after fluorescein sodium wrapped SiO2 nanoparticles,its structure is stable,did not change significantly.Fluorescence spectra showed that fluorescent silica composite nanoparticles have strong fluorescence and the maximum emission wavelength of fluorescein sodium occurred red shift;Light bleaching experiments further evidence:The compact fluorescent nanoparticles has better ability to resist photobleaching;Dye leakage experiments show SoF@SiO2 composite materials in aqueous solution in fluorescent stability is better,dye leakage is not obvious.Through the single factor experiment and response surface designs,to optimize the solid loading rate of bionic fluorescent silica adsorption of bovine serum albumin.Separately control pH,protein concentration,adsorption time three conditions,get the best optimization condition:PH 8.102,protein concentration is 1.056 mg/ml,adsorption time is 3.221 h.At this time,the solid loading ratio is the highest of bionic fluorescent silica to bovine blood BSA,is 77.520%.In the study of bionic fluorescent material adsorption of BSA fluorescence spectrum and interface features,mainly using SEM,EDS,XRD,FT-IR,PL,etc analysis instrument.SEM can clearly see on the bionic fluorescent material adsorb many bovine serum albumin;EDS map can be seen that dopamine,bovine serum albumin successfully fuse on the silica nanoparticles;In FT-IR spectrum,with the advent of amide is indicative of bovine serum albumin fusion to the bionic fluorescent SiO2 surface successfully;XRD spectrum can be seen that after the bionic fluorescent SiO2 adsorption bovine serum albumin,crystal type of SiO2 have a little impact;PL spectrum can be seen that the addition of BSA made the bionic SiO2 of fluorescence intensity is reduced,the maximum absorption peak faint red shift happened,BSA and bionic fluorescent SiO2 interacted.The bionic fluorescence quenching material,Ksv and Kq along with the rise of temperature is reduced,Maximum absorption of biomimetic fluorescent SiO2 slightly red shift.Prove that BSA on bionic fluorescence quenching of SiO2 type is static quenching,ΔH°<0、0 ΔS°>0,calculated that the reaction type between BSA and bionic fluorescent SiO2 is mainly electrostatic interactions.